Words similar to tween-
Example sentences for: tween-
How can you use “tween-” in a sentence? Here are some example sentences to help you improve your vocabulary:
The membranes were blocked in TBST (10 mM Tris-HCl pH 8.0, 150 mM NaCl, 0.1% Tween-20) with 5% nonfat dry milk (w/v).
Membranes were blocked in 5% BLOT-QuickBlocker PBST buffer (plus 0.1% Tween-20), washed three times with PBST buffer (plus 0.1% Tween-20), incubated in primary antibody diluted in femto/TBST buffer in a tray for 90 min at room temperature (or overnight at 4°C), washed five times (as above), incubated in secondary antibody-HRP (horseradish peroxidase)-conjugate (goat anti-rabbit) as above, washed four times with femto/TBST buffer followed by transfer to detection buffer and detection reagent.
The membrane was blocked with 3% non-fat dry milk (Bio-Rad), 0.1% tween-20 in TBS (pH 7.4), then cut at molecular weight marker 45 kDa.
Electrophoresis was performed in SDS-polyacrylamide gels [ 35 ] using the indicated amount of total cell protein [ 36 ] . After electrophoresis, the proteins were transferred to nitrocellulose paper in buffer containing 25 mM Tris-HCl, 192 mM glycine, 20% v/v methanol, and 0.01% SDS, pH 8.5 [ 37 ] . Residual protein binding sites on the nitrocellulose were blocked by incubation for 30 minutes in TBST (25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.5% Tween-20) containing 4% non-fat dry milk.
14 M NaCl, 1% bovine serum albumin, 0.05% Tween-20, 10 mM phosphate buffer, pH 7.4), for 1-2 hrs at room temperature.
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