Example sentences for: tween-

How can you use “tween-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All membranes were blocked for 1 hour with 5% non-fat milk Tris Buffered Saline plus 0.1% Tween-20 (T-TBS) and incubated at least overnight at 4°C in primary antibody.

  • Aliquots of the enzyme solutions were removed over time, diluted and assayed for activity using 1 mM suc-AAPF-pNA substrate in 100 mM Tris pH 8.6 containing 0.0005% Tween-80.

  • After removal of the supernatant, nuclei were resuspended in 1 ml 0.1 M Na 2 B 4 O 7 (pH 8.5) for two minutes at room temperature, and washed with 1 ml 0.5% Tween-20/1% BSA/PBS.

  • The probe arrays were then stained by adding 80 μl staining solution (100 mM MES, 1 M NaCl, 0.05% Tween-20 with 2 mg/ml acetylated BSA (Sigma, St Louis, MO) and 10 μg/ml streptavidin R-phycoerythrin (Molecular Probes, Portland, OR) for 30 min at 25°C.

  • Separated proteins were electroblotted onto nylon membranes (Millipore Corp., Bedford, MA) which were then incubated for 2 h in a blocking solution containing 5% non-fat dry milk in phosphate buffered saline containing 0.02% Tween-20.


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