Example sentences for: tween

How can you use “tween” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The filter was blocked with 3% BSA in TBS with 0.1% Tween 20 (T-TBS) for 30 min and incubated with the anti-phosphotyrosine antibodies at a dilution of 1:1000.

  • The membranes were washed in Tris-buffered saline containing 0.05% Tween 20 (TBST), blocked by immersing the membrane in 0.5% casein in TBST for 1 h then incubated at 4°C overnight with affinity purified goat anti-ERβ N-19 antibody (Santa Cruz Biotechnology) diluted 1:4000 in blocking reagent.

  • After overnight incubation, excess IgG antibody from the serum was washed away by thrice transferring the pegs to troughs containing 14 ml of PBS containing 0.05 % Tween 20 (PBS-Tween) for 5 min each time.

  • After 2 h at 37°C the wells were washed and HRP labeled goat anti-rabbit IgG (diluted in 1% BSA - 0.25% gelatin - 0.05% Tween 20 in PBS pH 7.4; {250 ng/well}) was added.

  • After 3 days of incubation at 37°C, bacteria from the resulting colonies were harvested, suspended in Middlebrook 7H9 media containing 10 μg kanamycin/mL and 0.05% (v/v) Tween 80, and pooled for reinfection of THP-1 macrophages.


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