Example sentences for: tween

How can you use “tween” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • M. paratuberculosis ATCC19698 was grown in Middlebrook 7H9 broth (pH 6.0) supplemented with oleic acid albumin dextrose complex (Becton Dickinson Microbiology) and 0.05% Tween 80 and ferric mycobactin J (2 mg/L).

  • After washing in Tris buffered saline containing 0.1% Tween 20 (TBST, pH 7.4), blots were incubated for 30 minutes at room temperature with anti-IgG secondary antibody conjugated to horseradish peroxidase.

  • Exponentially growing cells on slides were fixed in 4% paraformaldehyde in PEM buffer (80 mM Pipes [pH 7.0], 5 mM EGTA and 2 mM MgCl 2 ) for 30 min, permeabilized in 0.5% Triton X-100 in PEM buffer at room temperature for 15 min and rinsed three times with TBS + 0.1% Tween 20, followed by incubation in 0.5% nonfat dry milk in TBS + 0.1% Tween 20 for 2 h at room temperature.

  • 05% Tween 20 at 37°C -55°C with or without 50% - 60% formamide.

  • The nitrocellulose membranes were blocked in PBS with 0.1% Tween and 5% dried milk for 30 min at room temperature, washed 1 × in PBS/0.


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