Words similar to ttbs

Example sentences for: ttbs

How can you use “ttbs” in a sentence? Here are some example sentences to help you improve your vocabulary:

The membranes were washed 3 times for 10 min each with TTBS, incubated in anti-mouse or anti-rabbit HRP (1:10,000) in 1% milk for 1 hr and washed as above, the bands visualized using the ECL (Amersham).
Samples were probed with 50 μg biotin-Cry1Aa and streptavidin-conjugated horseradish peroxidase for 1 hr each, with 45 min TTBS washes, and developed in DAB/Urea (BioRad).
The membranes were blocked for 1 h in 5% milk in TTBS and then incubated overnight at 4°C in primary antibody (1:50 anti-Coll I, anti-Coll III and anti-FN, 1:100 TIMP-1, 1:500 anti-MMP-1 and anti-TIMP-2, 1:1000 anti-TN), in 1% milk in TTBS.
For ligand blot assays, samples separated by SDS-PAGE (9%) were transferred to PVDF overnight, blocked with 5% dried milk in TTBS (50 mM Tris-HCl, 150 mM NaCl, 0.05% Tween 20, pH 7.5).