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Example sentences for: transformants

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We selected 25 Arg +transformants and analyzed them by Southern blotting for integration of the UAU1 cassette into one of the two CaCET1 chromosomal loci to yield the heterozygote CaCET1/cacet1::UAU1 configuration depicted in Figure 1. Briefly, genomic DNA was isolated from the 25 Arg +strains, then digested with Sca I (which cuts neither CaCET1 nor UAU1 ). The digests were resolved by agarose gel-electrophoresis and transferred to membranes, which were probed with a radiolabeled DNA corresponding to the 5' segment of CaCET1 (probe A in Figure 1).
Cell transformants were pooled and an aliquot was plated to determine the total number of transformants.
Of the 24 transformants, 11 had the M. smegmatis sigE gene disrupted by
The library was generated from about 4000 independent transformants.
Some isolates had more than one band, suggesting that some satellite sequences may be unstable in yeast cells, as has been previously reported [ 21 ] . Alternatively, the transformants may have acquired more than one YAC.