Words similar to trans-blot
Example sentences for: trans-blot
How can you use “trans-blot” in a sentence? Here are some example sentences to help you improve your vocabulary:
10 μl of the brush border membrane preparation was diluted in 90 μl of Laemmli buffer, boiled for 5 min and separated on a denaturing polyacrylamide gel (5 % stacking gel/10 % resolving gel) at 120 V over 2 hours [ 18 ] . The separated proteins were transferred onto nitrocellulose membrane using a Trans-blot instrument (Biorad, Hercules, CA) in glycine-methanol buffer following the manufacturer's suggestions.
Fifteen (15) μl of the samples were electrophoresed on a 7.5% SDS-polyacrylamide mini-gel for 1 h at 150 V. The proteins were transferred to a PVDF membrane (Amersham Life Sciences, Arlington Heights, IL) using a Mini Trans-blot Cell (Bio Rad Laboratories, Hercules, CA) at 50 mA.