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Example sentences for: tprs
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The crystal structure of the PP5 TPR domain revealed three TPRs followed by an extended α helix [ 24 ] , however Ppt1p was originally proposed to contain one additional TPR [ 2 ] . The pattern of proteolysis and sites of cleavage for Ppt1p are very similar to those seen with PP5 under these same conditions [ 19 ] . This indicates that Ppt1p also contains an accessible region linking the TPR and catalytic domains and is most consistent with the presence of three TPRs in Ppt1p.
2. Thus, all the TPRs of PfPP5 may satisfy the structural requirements of a TPR motif.
To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
The TPR motif was originally identified as a protein interaction module in the cell division cycle (CDC) proteins in yeast [ 36 37 ] but has since been shown to occur in proteins with diverse functions in a variety of species [ 9 ] . The number of the TPR domains, the spacing between them, and their placement in the primary structure of the protein appear variable, and the relative contribution of the individual TPRs in a given protein has not been studied.
A detailed sequence analysis of all the PfPP5 TPRs is offered below.
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