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Example sentences for: tprs
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The TPR motif is a degenerate, 34-amino acid repeat that is often found in tandem arrays, sometimes separated by spacer sequences [ 9 ] . We propose such a 22-residue spacer between the first two TPRs of PfPP5 (Fig.
Protein phosphatase 5 (PP5), a newer member of the PPP family, differs from the other Ser/Thr phosphatases in that it contains regulatory and (sub)cellular targeting functions within a single polypeptide [ 5 6 7 ] . While its catalytic core exhibits strong similarity to those of the other members of this family, its N-terminus consists of three tetratricopeptide repeats (TPRs) that are unique to the PP5 class.
To identify whether PfPP5 is actually expressed in the parasite, cell-free extracts of the asynchronous erythrocytic stage parasites were subjected to standard chromatographic procedures that had been previously optimized for human PP5 [ 18 ] . Studies of mammalian PP5 have revealed that the TPR domain might act as an auto-inhibitory domain of the phosphatase activity, and that tryptic digestion of PP5 causes loss of the TPRs and concomitant stimulation of activity [ 19 20 21 ] . As described under Materials and Methods, the purification was in fact monitored by assaying for a trypsin-activated phosphatase with the assumption that PfPP5 may behave like mammalian PP5 in this regard [ 18 20 21 ] . As mentioned, the activity peaks always coincided with a ~69 kDa band in parallel Western analyses of the chromatographic fractions.
2. Thus, all the TPRs of PfPP5 may satisfy the structural requirements of a TPR motif.
The crystal structure of the PP5 TPR domain revealed three TPRs followed by an extended α helix [ 24 ] , however Ppt1p was originally proposed to contain one additional TPR [ 2 ] . The pattern of proteolysis and sites of cleavage for Ppt1p are very similar to those seen with PP5 under these same conditions [ 19 ] . This indicates that Ppt1p also contains an accessible region linking the TPR and catalytic domains and is most consistent with the presence of three TPRs in Ppt1p.
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