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Example sentences for: total
How can you use “total” in a sentence? Here are some example sentences to help you improve your vocabulary:
In total, 21 of the 199 genes surveyed from the chromosome 1 and 13 QTL regions showed nonsynonymous changes in their coding regions between B6 and D2 animals (Table 1).
Twenty seven fibers total (three fibers from each of 9 equal quadrats) were randomly selected from the region on the in situ slide preparation and densitometric measurements of the VEGF mRNA signal within fibers were made.
In order to describe total diet we used composite scores, which are necessary for the evaluation of epidemiological associations [ 12 ] . We defined subjects who are "closer" to the Mediterranean type of diet using as cut-off points the median values of the monthly food consumption score as done by several investigators in the past [ 12 ] . Finally, alcohol consumption was measured by daily ethanol intake, in wineglasses (100 ml of 12% ethanol concentration).
Isolation of total cellular RNA using a urea/LiCl protocol and gel electrophoresis was performed as described previously [ 65 ] . Gels were transferred onto Amersham Hybond N+ nylon membranes in 20 × SSC and prehybridized in 4 × SSC, 1 × Denhardt's solution, 0.5% SDS for 2 h at 68°C.
Expression of Her2/neu is amplified in 30% of all breast tumors and data suggest that it might be a negative prognostic indicator [ 48 ] . Figure 9Ashows the levels of Her2/neu protein normalized for total protein in tumor and normal tissues.
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