Words similar to thioguanine
Example sentences for: thioguanine
How can you use “thioguanine” in a sentence? Here are some example sentences to help you improve your vocabulary:
F3 cells were grown for two passages in media containing 250 μg/ml 6-thioguanine.
Of the original 192 G418-resistant clones that were harvested only one, named F3, was also resistant to 6-thioguanine and sensitive to growth in HAT medium (fig 3b).
Following transfection of this plasmid into R1 cells 192 clones that were resistant to G418 were collected and grown in the presence of 6-thioguanine to select for loss of Hprt activity.
Furthermore, fig 3bshows that these cells had become G418 and 6-thioguanine sensitive confirming that the neo gene had indeed been replaced with hprt -coding sequences in the F3 cells.