Words similar to thdp

Example sentences for: thdp

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Time course experiments indicated ThDP uptake was linear for at least 20 minutes (data not shown), and uptake was linear with the amount of mitochondrial protein added (inset of fig.
Although the estimated concentration, in mice and human cells, of intracellular ThDP is about 30 μM, much of this is found in a low turnover pool representing enzyme-bound ThDP [ 4 17 ] . The estimated concentration of free cytosolic ThDP available for intracellular transport is about 10% of the total concentration and thus 2 to 3 μM [ 4 17 18 ] . Hence the physiological significance of the mitochondrial ThDP uptake just decribed is uncertain.
The apparent affinity for ThDP characteristic of the high affinity uptake component was essentially identical for all cell types examined, however variation was seen in transport capacity, as revealed by substantially different values for V max (table 1).
However, this is not the case as glyB cells that lack the mitochondrial folate transporter were found to take up ThDP with high affinity kinetics similar to that of mitochondria of human cells.
Uptake of thiamine and ThDP by mitochondria was determined by a rapid filtration procedure [ 26 28 ] . Incubations were performed at 37°C by rapidly mixing 30 μl of mitochondrial suspension (ca.