Example sentences for: tg

How can you use “tg” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • pΔHprt was constructed by introducing a 2.5 Kb BamHI/HindIII fragment of 5' hprt genomic DNA (E14TG2a origin) from the plasmid pMP8 into the Hpa1 site of pOS.DUP/DEL [ 15 ] . The ends of the fragments were made blunt using Klenow.

  • Nagy et al demonstrated that embryos and mice could be derived solely from R1 ES cells by aggregating them with tetraploid embryos [ 7 8 ] . Although we were able to repeat these data using R1 ES cells we were unable to generate midgestation embryos from E14TG2a-derived strains of hprt negative ES cell lines using the same approach [ 9 10 11 ] . We believed it was unlikely that Hprt itself was required to generate ES cell-derived embryos because hprt knockout mice are viable and fertile [ 12 ] . E14TG2a-derived ES cell lines contain a large spontaneous deletion encompassing 55 kb of the hprt locus [ 13 ] . Therefore, it seemed possible that sequences within this region were necessary to maintain ES cell pluripotency.

  • However, tumors from Wnt-1 TG, Pten +/+ mice have low apoptotic rates (40 ± 16 per digital camera field using a 20x objective) and high proliferation rates (53% ± 11).

  • The majority of tumors in Wnt-1 TG, Pten +/- mice had lost the wild type allele of Pten , suggesting that the complete inactivation of Pten function provides an additional growth advantage to these tumors.

  • [ 22 ] developed transgenic (TG) mice overexpressing rat ABP.


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