Example sentences for: thawing

How can you use “thawing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • After 16 h post-infection, cells and media were collected, virus was released by freezing and thawing and virus yields determined by plaque assay.

  • Whole cell extraction was performed by lysis of cells in 250 mM Tris-Cl pH 7.5, three cycles of freezing and thawing and a subsequent centrifugation step for 5 min at 13,000 rpm at 4°C.

  • After thawing at room temperature, serum samples were diluted 1:10 with PBS.

  • By contrast, moderately freeze-tolerant species—those that can survive only a few degrees of freezing—appear to need little preparation for a freezing event and seem perfectly prepared to continue with their routine activities immediately after thawing.

  • The cell lines were mycoplasma free and cells were utilized up to the tenth passage before thawing frozen stock cells from liquid N 2 .


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