Example sentences for: thawed

How can you use “thawed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Samples were thawed, dialyzed against phosphate-buffered saline, and centrifuged again; the total sample volume was then applied to a column of immunoaffinity-purified antibody covalently bound to a Affi-Gel 15 matrix, and eluted with 0.1 M sodium citrate in 0.3 M NaCl, pH 3.0.

  • In brief, 2 × 10 8PBMC were thawed, washed twice in a 50:50 mixture of OPTI-MEM and Plasbumin (Bayer, West Heven, CT) and incubated for 3 h in a T175 flask in 50 ml serum-free OPTI-MEM in the presence or absence of 6,000 IU IL-2 per ml OPTI-MEM.

  • Stock vanadyl and nucleotide solution were thawed on ice, and mixed at 1:1 molar ratio by vigorous stirring.

  • Fish should be fed as much as they will eat at least once a day with live or frozen brine shrimp or dry food (frozen food should be completely thawed before use).

  • Both the LPS stock solution [10 ng/ml] and dilutions were stored at -80°C, thawed prior to each experiment and discarded after use.


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