Example sentences for: thawed

How can you use “thawed” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For each experiment described below the cells were thawed, cultured and passed at least once.

  • Brush border membrane vesicles were prepared as described by deRooij [ 11 ] . Briefly, frozen ileal tissue fragments (20-25 mg) were thawed and homogenized in 100 μl of isotonic buffer (300 mM mannitol, 12 mM Tris-HCl, pH 7.1) using a Polytron homogenizer (Kinematica AG, Littau, Switzerland).

  • Our electrospray mass spectrometry results clearly showed that undegraded domoic acid was present, demonstrating the stability of domoic acid in the aqueous solutions that had been frozen at -80°C and thawed prior to use in the experiments.

  • At a later date, frozen livers, colon or tumor were thawed and homogenized individually at 4°C in 280 mM mannitol with 10 mM HEPES buffer with 0.01% BHT using a Polytron homogenizer.

  • Just prior to use, an aliquot was thawed and 2 μl of stock solution were diluted into 10 ml of PBS.


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