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Example sentences for: tfiih
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The human FHIT gene, located at the chromosome 3 fragile site FRA3B, is inactivated early in the development of many tumors [ 1 ] . Murine Fhit is also located at a fragile site [ 2 3 ] and mice heterozygous for disruption of Fhit , given low intragastric doses of the mutagen N-nitrosomethylbenzylamine, develop stomach and sebaceous tumors [ 4 ] that can be prevented by viral Fhit expression [ 5 ] . Fhit, a dimer of 147 amino acid subunits, is a member of the histidine triad (HIT) superfamily of nucleotide hydrolases and transferases [ 6 7 ] . Members of the Hint branch of the HIT superfamily are found in all forms of life [ 8 ] . The S. cerevisiae Hint homolog, Hnt1, and rabbit Hint possess adenosine monophosphoramidase activity that functions in yeast to positively regulate function of Kin28, Ccl1 and Tfb3, which constitute the kinase component of general transcription factor TFIIH [ 9 ] . A new Hint related protein, Aprataxin, is mutated in individuals with ataxia with oculomotor apraxia [ 10 11 ] and has a yeast homolog termed Hnt3 [ 9 ] . Members of the Fhit branch of the HIT superfamily have been found in fungi [ 12 13 ] , animals [ 2 14 15 ] and plants [ 7 ] and hydrolyze diadenosine tetraphosphate, diadenosine triphosphate and other 5'-5"'-dinucleoside polyphosphates.
Next, the GTF TFIIH unwinds the DNA, opens the transcription bubble, and phosphorylates serines at position 5 in the C-terminal domain (CTD) of the RPB1 subunit of RNAPII (reviewed in [2]).
Where indicated recombinant NFATp proteins were pre-incubated with promoter DNA for five min at 30°C prior to the addition of the remaining general transcription factors (TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH) and RNA polymerase II.
Loss of this enzymatic activity renders yeast cells temperature-sensitive for growth on galactose medium and hypersensitive to mild mutations in the yeast homolog of mammalian Cdk7, that is, Kin28, the kinase component of general transcription factor TFIIH [ 30].
To this end we used an in vitro human RNA polymerase II transcription system reconstituted from highly purified native (n) and recombinant (r) proteins, including (r)TFIIA, (r)TFIIB, (n)TFIID, (r)TFIIE, (r)TFIIF, (n)TFIIH, and (n)RNA polymerase II.
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