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Example sentences for: tfiid

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RNA polymerase II (pol II) transcription factor TFIID comprises the TATA-binding protein (TBP) and a set of TBP-associated factors (TAF II s) [ [ 1 2 3 ] ]. At least 12 TAF II s have been identified in TFIID and cloning of their cDNAs has shown an evolutionary conservation of TAF II s from yeast to mammals [ [ 4 5 6 7 ] ]. TAF II s are not only components of the TFIID complex, but a subset of TAF II s are also found in the SAGA, PCAF, TFTC/STAGA complexes which lack TBP [ [ 8 9 10 11 12 ] ].
To determine whether components of basal transcription factor TFIID were subject to regulation during F9 cell differentiation, immunoblotting with monoclonal antibodies against TBP and a number of TAF II s, was used to monitor the levels of the respective proteins in differentiated cell extracts (see Materials and Methods).
The central component of PIC is the general transcription factor (GTF) TFIID, which contains the TATAbox- binding protein (TBP) and 12 to 15 TBP-associated factors (TAFs).
This process is specific for TBP and TAF II 135 as it is not observed with the other TFIID components analysed, and is induced concomitantly with that of the RARγ2.
Therefore, there is a selective depletion of these two TFIID components in extracts from differentiating cells.