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Example sentences for: tfiid
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RNA polymerase II (pol II) transcription factor TFIID comprises the TATA-binding protein (TBP) and a set of TBP-associated factors (TAF II s) [ [ 1 2 3 ] ]. At least 12 TAF II s have been identified in TFIID and cloning of their cDNAs has shown an evolutionary conservation of TAF II s from yeast to mammals [ [ 4 5 6 7 ] ]. TAF II s are not only components of the TFIID complex, but a subset of TAF II s are also found in the SAGA, PCAF, TFTC/STAGA complexes which lack TBP [ [ 8 9 10 11 12 ] ].
Aside from induction of ICER, Tat is capable of forming complex (es) with a component of TFIID, TAF II 250 [ 24 ] and Tip60 [ 25 ] both of which contain histone acetyltransferase (HAT) activity.
Thus, while the overall level of TFIID is diminished through the reduction in TBP and TAF II 135, the relative stoichiometry of the remaining TFIID is not significantly altered.
To determine whether components of basal transcription factor TFIID were subject to regulation during F9 cell differentiation, immunoblotting with monoclonal antibodies against TBP and a number of TAF II s, was used to monitor the levels of the respective proteins in differentiated cell extracts (see Materials and Methods).
Where indicated recombinant NFATp proteins were pre-incubated with promoter DNA for five min at 30°C prior to the addition of the remaining general transcription factors (TFIIA, TFIIB, TFIID, TFIIE, TFIIF, and TFIIH) and RNA polymerase II.
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