Example sentences for: swy

How can you use “swy” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Crossing a nup170-GFP:URA3 haploid isolate to SWY1695 generated doubly tagged GFP-nic96 nup170-GFP parental strains.

  • Mutagenesis of SWY2089 and SWY2090 with ethylmethane sulfonate (EMS; Sigma, St. Louis, MO) was performed as described with the following modifications [ 67 ] . First, cells were mutagenized for 60, 75, and 90 minutes, followed by plating a fraction of the culture to determine cell viability at 23°.

  • Sequence for the mutant sec13 and sec23 alleles were cloned using the same oligonucleotide pairs with genomic DNA from SWY2324 or SWY2325, serving as template.

  • GFP-tagged strains of other known mutants were made by crossing to either parental strain SWY2089 or SWY2090.

  • The resulting fragment was gel purified and transformed into SWY519.


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