Words similar to swy

Example sentences for: swy

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Crossing a nup170-GFP:URA3 haploid isolate to SWY1695 generated doubly tagged GFP-nic96 nup170-GFP parental strains.
The resulting fragment was gel purified and transformed into SWY519.
Sequence for the mutant sec13 and sec23 alleles were cloned using the same oligonucleotide pairs with genomic DNA from SWY2324 or SWY2325, serving as template.
Mutagenesis of SWY2089 and SWY2090 with ethylmethane sulfonate (EMS; Sigma, St. Louis, MO) was performed as described with the following modifications [ 67 ] . First, cells were mutagenized for 60, 75, and 90 minutes, followed by plating a fraction of the culture to determine cell viability at 23°.
GFP-tagged strains of other known mutants were made by crossing to either parental strain SWY2089 or SWY2090.