Words similar to swy

Example sentences for: swy

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The SWY2324, SWY2325, SWY2332, and SWY2333 strains were transformed with a LEU2/CEN yeast genomic library (ATCC, Manassas, VA).
Sequence for the mutant sec13 and sec23 alleles were cloned using the same oligonucleotide pairs with genomic DNA from SWY2324 or SWY2325, serving as template.
GFP-tagged strains of other known mutants were made by crossing to either parental strain SWY2089 or SWY2090.
The resulting fragment was gel purified and transformed into SWY519.
Mutagenesis of SWY2089 and SWY2090 with ethylmethane sulfonate (EMS; Sigma, St. Louis, MO) was performed as described with the following modifications [ 67 ] . First, cells were mutagenized for 60, 75, and 90 minutes, followed by plating a fraction of the culture to determine cell viability at 23°.