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Example sentences for: sw-
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Effects of CDDO in SW-1353 cells
In a previous study, Northern blot analysis showed that treating SW-1353 cells with 300 nM CDDO for 24 hours before treatment with IL-1 inhibited the expression of MMP-1 and MMP-13 [ 23 ] . In the present study we used quantitative real-time RT-PCR to examine the action of CDDO on the expression of MMP-1 and MMP-13 in SW-1353 cells and in two primary cultures: human primary chondrocytes and OA synovial fibroblasts.
To examine further the role of Bcl-3 in IL-1-induced MMP-1 and MMP-13 expression, we used SW-1353 cells stably transfected with a Bcl-3 expression construct.
Human SW-1353 chondrosarcoma cells were purchased from the American Type Culture Collection (Rockville, MD, USA).
We found that SW-1353 cells expressed very low basal levels of MMP-1 and MMP-13, and that these were not significantly affected by incubation with CDDO (Fig.
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