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Example sentences for: sw-

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SW-1353 cells (normal or stably transfected with pBkRSV-Bcl-3), human primary chondrocytes, or synovial fibroblasts were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA), penicillin/streptomycin and L-glutamine (Cellgro; Mediatech, Herndon, VA, USA) before use in experiments.
As was observed with SW-1353 cells, MMP-13 expression was significantly decreased when CDDO was added simultaneously with the cytokine: we observed a decrease of 67% ( P < 0.1).
However, a dose of 5 μM caused apoptosis in SW-1353 cells, as seen in lower cell numbers, changed morphology and an increased number of fluorescing cells.
We found that SW-1353 cells expressed very low basal levels of MMP-1 and MMP-13, and that these were not significantly affected by incubation with CDDO (Fig.
Previous work suggested that preincubation with CDDO was required for the effective inhibition of IL-1-induced MMP gene expression in SW-1353 cells [ 23 ] . To quantify the effect of altering the period of CDDO preincubation, 300 nM CDDO was added to SW-1353 cells simultaneously with IL-1, or 24 hours before treatment with the cytokine.