Words similar to suspension
Example sentences for: suspension
How can you use “suspension” in a sentence? Here are some example sentences to help you improve your vocabulary:
P. falciparum parasite cells (W2 strain) were cultured as described [ 54] with slight modifications: 2% suspension of purified human red blood cells in RPMI1640 media supplemented with 0.25% AlbumaxI (GIBCO/Invitrogen, San Diego, CA), 2 g/l sodium bicarbonate, 0.1 mM hypoxanthine, 25 mM HEPES pH 7.4, and 50 μg/I gentamycin.
Following cell thaw, NSs were expanded in suspension culture, and in each case were used between passages 5 and 7. Undifferentiated human NS cells (stage 1) were cultured and expanded in medium containing X-VIVO15 (Cambrex, Walkersville, Maryland, United States), N2 supplement (Invitrogen, Carlsbad, California, United States), 2 μg/ml heparin, 10 ng/ml leukemia inhibitory factor (Chemicon, Temecula, California, United States), 20 ng/ml epidermal growth factor (R&D Systems, Minneapolis, Minnesota, United States), and 20 ng/ml fibroblast growth factor-2 (R&D Systems), and they were frozen after enzymatic dissociation.
To obtain nuclei, (4-6) × 10 7cells of each cell line grown in the presence and absence of 2.5 μM SAHA were washed twice in PBS, followed by suspension in 0.3 ml nuclear buffer consisting of 2 mM MgCl 2 , 5 mM K 2 HPO 4 , 0.1 mM EDTA, 1 mM PMSF, 20 μg/ml aprotinin, 20 μg/ml leupeptin, 0.1 mM Na 3 VO 4 and 5 mM β-glycerophosphate.
Aliquots from this final suspension were subjected to centrifugation and washed twice immediately prior to fluorescence measurement.
To prepare cell envelopes, cells were suspended in 0.01 M HEPES, pH 7.4 and sonicated on ice by using a Branson Sonifier (small tip, setting 7) [ 44 ] . The suspension was centrifuged at 12,000 x g for 2 minutes at 4°C to remove unbroken cells and debris.
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