Words similar to suspension

Example sentences for: suspension

How can you use “suspension” in a sentence? Here are some example sentences to help you improve your vocabulary:

HEL cells were dislodged from the flask with a trypsin-EDTA solution, washed with DEPC-treated PBS, and then resuspended in 6 ml of ice-cold resuspension buffer [10 mM Tris-HCl (pH 7.5), 10 mM KC1, 5 mM MgCl 2 ]. Six ml of hot (70°C) lysis buffer [20 mM Tris-HCl (pH 7.4), 0.4 M NaCI, 40 mM EDTA, 1% β-mercaptoethanol, 1% SDS] and 1 ml of citrate-buffered phenol (pH 4.0) were added to each cell suspension.
The kidneys were perfused with 10 ml of ice cold normal saline, followed by 10 ml of a 1% suspension of magnetized iron oxide (Fe 3 O 4 , Aldrich) in normal saline.
For preparation of donors in G0/G1 phase, the cells were allowed to grow to confluency by culturing for 6 days and a single cell suspension was prepared by standard trypsinization.
The pTX-MKC2 plasmid was therefore transfected into 3xALA myosin II cells, which are resistant to myosin filament hyperphosphorylation and disassembly due to elimination of phosphorylation target sites in the myosin tail [ 24 ] . The resultant 3xALA/pTX-MKC2 cells could be propagated in suspension culture even after selection for elevated expression in 40 μg/ml G418.
As shown in Figure 1, this transfer to suspension culture resulted in elevated expression of PTEN protein in each cell line.