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Example sentences for: suspended

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Lysozyme-treated cells were washed in 1 ml of Buffer NT (1 M NaCl, 50 mM Tris pH 7.5) and were suspended in Buffer NT to a final volume of 200 μl.
One tendon clip was attached to a force transducer hook and the other to a Cambridge motor arm (model 300B), allowing the fiber bundle to be suspended horizontally and adjusted to a specific sarcomere length, as desired during the experiment.
3 × 10 5) suspended in 600 ul media were incubated at 37°C overnight in 6% CO 2 with anti-CD3 (1:500 dilution of acetates fluid) and PMA (2.
Addition of MTX to fura-2 -loaded BAECs, suspended in a cuvette in normal Ca 2+-containing buffer at 37°C, produced a time-dependent increase in [Ca 2+] i (Fig 1, upper panel ). Addition of either U73122 or U73343, the inactive analog, ~3 min before MTX produced a concentration-dependent inhibition of the change in [Ca 2+] i . U73343 exhibited a 3-fold greater potency compared to U73122 (Fig 1, insets ). Since U73343 has no significant effect on PLC over the concentration range employed [ 14 25 26 27 ] , these results suggest that inhibition of the MTX-induced response is independent of PLC.
The cells were stained with anti-CD14 mAb conjugated with FITC, and they were suspended in 110 μl PBS containing 3% FBS.