Words similar to superscriptii
Example sentences for: superscriptii
How can you use “superscriptii” in a sentence? Here are some example sentences to help you improve your vocabulary:
For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).
Although these numbers are higher than the 1 in 15,000 figure reported for SuperScriptII (Taurai Nenguke, personal communication), the in vitro assay used to obtain this error rate is based on assaying a single site for mutations that revert an amber codon.
Two micrograms of the extracted RNA was treated with 2 U DNaseI to eliminate DNA, and reverse transcribed with 200 U SuperScriptII reverse transcriptase at 42°C for 70 min using oligo dT primers in accordance with the manufacturer's instructions.
Trizol reagent, deoxyribonucleaseI, and SuperScriptII reverse transcriptase were purchased from Gibco BRL (Rockville, MD).
Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).