Example sentences for: superscriptii

How can you use “superscriptii” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • For RT-PCR, 1 μg of total RNA was reverse-transcribed into cDNA using SuperscriptII reverse transcriptase (Invitrogen, Carlsbad, CA).

  • Trizol reagent, deoxyribonucleaseI, and SuperScriptII reverse transcriptase were purchased from Gibco BRL (Rockville, MD).

  • Small samples (2 μg) from each RNA fraction were reverse transcribed and radiolabeled (2 μg oligo dT, 1x First Strand buffer, 5 mM DTT, 75 U SuperscriptII [Life Technologies], 20 mM dNTP mix containing dATP, dGTP, dTTP and 100 mCi 33P-dCTP (ICN Radiochemicals).

  • Two micrograms of the extracted RNA was treated with 2 U DNaseI to eliminate DNA, and reverse transcribed with 200 U SuperScriptII reverse transcriptase at 42°C for 70 min using oligo dT primers in accordance with the manufacturer's instructions.

  • Although these numbers are higher than the 1 in 15,000 figure reported for SuperScriptII (Taurai Nenguke, personal communication), the in vitro assay used to obtain this error rate is based on assaying a single site for mutations that revert an amber codon.


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