Words similar to superscript
- sunday
- suny
- sup
- super
- super-standard
- superadult
- superamine
- superanova
- superase-in
- superasin
- supereditors
- superfect
- superman
- superposition
- superpower
- superproblems
- superpundit
- superritzy
- supersaturated
- supersaturation
- supersaver
- supersccript
- superscript
- superscript-
- superscriptii
- superscripts
- supersecret
- supersede
- superseded
- supersedes
- superseding
- supersensitive
- supershift
- supersignal
- supervisor
- supplemental
- supplementary
- supplied
Example sentences for: superscript
How can you use “superscript” in a sentence? Here are some example sentences to help you improve your vocabulary:
The cDNA was synthesized from 16 μg total RNA using 5 μg random 8-base oligonucleotides (Sigma GenoSys) and Superscript II™ reverse transcriptase (Invitrogen).
Reverse transcription of 2 μg of total RNA was performed after Dnase I digestion (Roche Diagnostics, Indianapolis, IN) with the Superscript™ II RNase H-Reverse Transcriptase (GibcoBRL Life Technologies) protocol for cDNA synthesis using 500 ng of random hexamers.
Reverse transcription was performed in the presence of 500 μM each of dATP, dCTP, and dGTP, 200 μM 5-aminoallyl-dUTP (Sigma), 300 μM dTTP, 1 × first-strand buffer, 10 mM dithiothreitol, and 400 U Superscript II (Life Technologies) in 40-μl reactions at 42°C for 3 h to overnight.
To generate labeled single-stranded cDNA target, 10 μg total RNA from rat heart or brain (Clontech, Palo Alto, CA) was reverse transcribed for 2-3 h at 42°C in the presence of 6 μg random primers (Invitrogen/Life Technologies), 1x first-strand synthesis buffer (Invitrogen/Life Technologies), 10 mM DTT, dNTP mix (25 mM dATP, 25 mM dCTP, 25 mM dGTP, 15 mM dTTP, 10 mM amino allyl-dUTP), and 200 units Superscript II reverse transcriptase (Invitrogen/Life Technologies).
Briefly, total cellular RNA was prepared with Qiagen RNeasy reagents, and 5 μg samples were used to prepare T7-dT-primed double-stranded cDNA (SuperScript Choice, Invitrogen).
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