Example sentences for: superscript

How can you use “superscript” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • In short, double-stranded cDNA was synthesized from the total RNA using the SuperScript II kit from Invitrogen and the Affymetrix T7-(dT) 24 primer, which contains a T7 promoter attached to a poly-dT sequence 5'-GGCCAGTGAATTGTAATACGACTCACTATAGGGAGGCGG-(dT) 24 -3'.

  • 1 M), d(GAT)TP (25 mM), dCTP (1 mM), Cy3-dCTP (2 mM)(Amersham, Piscataway, NJ) and Superscript RT II (200 units) were added and incubated at room temperature for 10 minutes and then at 42°C for 2 hours.

  • Reverse transcription was carried out in a 20 μl volume containing 1 μg total RNA, 1 μg oligdT25- T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGG -CGATT-3') primer, 1 μg template switch primer (5'-AAGCAGTGGTAACAACGC -AGGGACCGGG-3'), 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL) 1 μl 10 mM dNTPs, 1 μl SUPERase-in, (Amtion, Austin, TX) and 200 u Superscript II reverse transcriptase (Gibco- BRL).

  • Briefly, total cellular RNA was prepared with Qiagen RNeasy reagents, and 5 μg samples were used to prepare T7-dT-primed double-stranded cDNA (SuperScript Choice, Invitrogen).

  • The superscript Tdesignates transposition.


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