Words similar to superscript
- sunday
- suny
- sup
- super
- super-standard
- superadult
- superamine
- superanova
- superase-in
- superasin
- supereditors
- superfect
- superman
- superposition
- superpower
- superproblems
- superpundit
- superritzy
- supersaturated
- supersaturation
- supersaver
- supersccript
- superscript
- superscript-
- superscriptii
- superscripts
- supersecret
- supersede
- superseded
- supersedes
- superseding
- supersensitive
- supershift
- supersignal
- supervisor
- supplemental
- supplementary
- supplied
Example sentences for: superscript
How can you use “superscript” in a sentence? Here are some example sentences to help you improve your vocabulary:
Reverse transcription was carried out in a 20 μl volume containing 1 μg total RNA, 1 μg oligdT25- T7 (5'-AAACGACGGCCAGTGAATTGTAATACGACTCACTATAGGG -CGATT-3') primer, 1 μg template switch primer (5'-AAGCAGTGGTAACAACGC -AGGGACCGGG-3'), 4 μl first-strand reaction buffer, 2 μl 10 mM dithiothreitol (DTT; Gibco-BRL) 1 μl 10 mM dNTPs, 1 μl SUPERase-in, (Amtion, Austin, TX) and 200 u Superscript II reverse transcriptase (Gibco- BRL).
cDNA was synthesized with oligo (dT)12-18 using Superscript First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA).
Total RNA (2 μg) was reverse transcribed using SuperScript II Reverse transcriptase (Invitrogen) and random hexamers (Amersham Biosciences).
The primers were annealed to template at 25°C for 10 min, and the reaction were incubated at 53°C for 1 h in the presence of Superscript Reverse transcriptase, 5 mM dNTP mix and RN'ase Out inhibitor.
Total RNA was obtained from mouse embryonic tissue or adult mouse organs and reverse transcription carried out as described [ 34 ] . For each tissue analysis, PCR was carried out in a 25 μl reaction volume using 1 μl of the first-strand cDNA reaction, according to the Superscript Preamplification System for First Strand cDNA Synthesis (Invitrogen).
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