Words similar to superdex
Example sentences for: superdex
How can you use “superdex” in a sentence? Here are some example sentences to help you improve your vocabulary:
The major peak of aromatic aminotransferase was pooled and subjected to Superdex gel filtration as described above.
The fractions were concentrated by an Ultrafree-15 centrifugal filter device (Millipore Corporation), and loaded onto an FPLC-connected Superdex 75 HR 10/30 (Pharmacia Biotech) column which had been equilibrated with 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT and 150 mM NaCl.
Cry1Aa binding ability was also checked by slot blotting fractions to PVDF membrane and probing with biotinylated Cry1Aa [ 26 ] . Fractions with Cry1Aa-binding ability and APN enzymatic activity were concentrated to 2 ml volumes and loaded on a Superdex 200 size-exclusion column (120 ml bed volume) using Hepes-buffered saline (HBS; 10 mM Hepes, 150 mM NaCl, 3.4 mM EDTA, pH 7.4) as running buffer.
The molecular mass of the native enzyme was determined by gel filtration using an FPLC-connected Superdex 75 HR 10/30 column (Pharmacia Biotech).
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