Words similar to supercoiled

Example sentences for: supercoiled

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Partial relaxation of the input supercoiled DNA was detected when Top67 was mixed with the ZD domain prior to addition of DNA.
GST-BTBD2 slightly inhibited the supercoil relaxation activity of TOP1, increasing the time required to completely relax the supercoiled DNA by about two-fold as compared with GST alone (Fig.
Based on these results, we propose a model for the relaxation of supercoiled DNA by E. coli topoisomerase I (Figure 7) modified from previous versions that have a number of common features but differ most significantly in the role of the Zn(II) binding domain [ 2 4 21 29 30 ] . In this model, the subdomains in Top67 is responsible for interacting with the G-strand of DNA both upstream and downstream of the cleavage site.
As reported previously [ 11 ] , the N-terminal transesterification domain Top67 by itself did not exhibit any relaxation activity when assayed with negatively supercoiled plasmid DNA (Figure 2a).
Protein-free plasmid DNA was purified using Qiagen maxiprep columns (San Diego, CA) and contained predominantly supercoiled plasmid with no contaminating RNA or low molecular weight contaminants.