Words similar to supercoiled

Example sentences for: supercoiled

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These negatively supercoiled plasmids were treated with S1 nuclease or mock-treated for 5 min.
As reported previously [ 11 ] , the N-terminal transesterification domain Top67 by itself did not exhibit any relaxation activity when assayed with negatively supercoiled plasmid DNA (Figure 2a).
Protein-free plasmid DNA was purified using Qiagen maxiprep columns (San Diego, CA) and contained predominantly supercoiled plasmid with no contaminating RNA or low molecular weight contaminants.
Top67 and the ZD domains at different concentrations were mixed and incubated at 37°C for 10 min before addition to the 0.3 μg of supercoiled plasmid DNA in 20 μl of 10 mM Tris-HCl pH 8.0, 2 mM MgCl 2 , 0.1 mg/ml gelatin.
Based on these results, we propose a model for the relaxation of supercoiled DNA by E. coli topoisomerase I (Figure 7) modified from previous versions that have a number of common features but differ most significantly in the role of the Zn(II) binding domain [ 2 4 21 29 30 ] . In this model, the subdomains in Top67 is responsible for interacting with the G-strand of DNA both upstream and downstream of the cleavage site.