Words similar to supercoiled

Example sentences for: supercoiled

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GST-BTBD2 slightly inhibited the supercoil relaxation activity of TOP1, increasing the time required to completely relax the supercoiled DNA by about two-fold as compared with GST alone (Fig.
A substrate with both single-stranded and double-stranded regions was constructed to mimic such junction in negatively supercoiled DNA.
Based on these results, we propose a model for the relaxation of supercoiled DNA by E. coli topoisomerase I (Figure 7) modified from previous versions that have a number of common features but differ most significantly in the role of the Zn(II) binding domain [ 2 4 21 29 30 ] . In this model, the subdomains in Top67 is responsible for interacting with the G-strand of DNA both upstream and downstream of the cleavage site.
As reported previously [ 11 ] , the N-terminal transesterification domain Top67 by itself did not exhibit any relaxation activity when assayed with negatively supercoiled plasmid DNA (Figure 2a).
Partial relaxation of the input supercoiled DNA was detected when Top67 was mixed with the ZD domain prior to addition of DNA.