Example sentences for: supercoiled

How can you use “supercoiled” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Protein-free plasmid DNA was purified using Qiagen maxiprep columns (San Diego, CA) and contained predominantly supercoiled plasmid with no contaminating RNA or low molecular weight contaminants.

  • Partial relaxation of the input supercoiled DNA was detected when Top67 was mixed with the ZD domain prior to addition of DNA.

  • A substrate with both single-stranded and double-stranded regions was constructed to mimic such junction in negatively supercoiled DNA.

  • They have large double-stranded, circular, supercoiled, DNA genomes of 100-180 kb and are characterized by the occlusion of their virions in crystalline protein matrices.

  • Reiterated sequence elements are often observed to form an unwound S1 nuclease-sensitive DNA conformation, termed anisomorphic or left-handed DNA, when present in negatively supercoiled plasmids [ 22 24 ] . Nuclease-sensitive secondary structures within the HSV origin of replication are believed to be recognized by the viral origin binding protein, and mutations that reduce this complex formation in vitro also reduce viral replication, thereby indicating that localized torsional strain may occur during viral DNA replication [ 1 ] . The


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