Words similar to stui
Example sentences for: stui
How can you use “stui” in a sentence? Here are some example sentences to help you improve your vocabulary:
To determine which of these potential transcription factor binding sites is responsible for regulating normal CD5 expression, we performed site-directed mutagenesis on the CCAAT (mutC), κE2 (mutK) and Ets (mutE) sites from the StuI B reporter construct.
However, the deletion of an additional 43 bp (from StuI to HincII site at -172) reduces the reporter activity by more than five-fold to basal level, which is somewhat higher than the background level obtained with a promoterless luciferase construct (GLB = pGLBasic).
To determine whether either the CD5X or the CD5Y region can affect the full level of transcription controlled by the 43 bp CD5 regulatory promoter, we generated the mutations described above in the CD5X and CD5Y regions in the StuI B reporter construct.
To understand the effect of Ets-1 on CD5 expression, we co-transfected EL4 cells with increasing amounts of Ets-1 expression plasmid (pEVRF-ets-1) along with a StuI B reporter construct containing either the wild type (S) or mutated Ets binding site (E).
Mutagenized constructs based on StuI B or HincII plasmid are described in the site-directed mutagenesis section.
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