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Example sentences for: stui

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These deletion constructs define two regions as important for CD5 expression: a minimal/core promoter region (HincII to HinfI site) that is required for basal activity and a regulatory region necessary for the full CD5 expression (StuI to HincII) site in resting T cells.
As figure 4shows, the luciferase reporter activity from the wild type StuI B reporter construct increased in a dose-dependent fashion with increasing amounts of Ets-1 expression plasmid (lanes 1-4).
However, the deletion of an additional 43 bp (from StuI to HincII site at -172) reduces the reporter activity by more than five-fold to basal level, which is somewhat higher than the background level obtained with a promoterless luciferase construct (GLB = pGLBasic).
To understand the effect of Ets-1 on CD5 expression, we co-transfected EL4 cells with increasing amounts of Ets-1 expression plasmid (pEVRF-ets-1) along with a StuI B reporter construct containing either the wild type (S) or mutated Ets binding site (E).
The mutC, mutK, mutE, Xmut, and Ymut mutagenesis primers were first PCR amplified with GL2 primer (5' CTT TAT GTT TTT GGC GTC TTC CA 3') using StuI B plasmid as template to generate megaprimers.