Words similar to stui

Example sentences for: stui

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To determine whether either the CD5X or the CD5Y region can affect the full level of transcription controlled by the 43 bp CD5 regulatory promoter, we generated the mutations described above in the CD5X and CD5Y regions in the StuI B reporter construct.
To understand the effect of Ets-1 on CD5 expression, we co-transfected EL4 cells with increasing amounts of Ets-1 expression plasmid (pEVRF-ets-1) along with a StuI B reporter construct containing either the wild type (S) or mutated Ets binding site (E).
To determine which of these potential transcription factor binding sites is responsible for regulating normal CD5 expression, we performed site-directed mutagenesis on the CCAAT (mutC), κE2 (mutK) and Ets (mutE) sites from the StuI B reporter construct.
The mutC, mutK, mutE, Xmut, and Ymut mutagenesis primers were first PCR amplified with GL2 primer (5' CTT TAT GTT TTT GGC GTC TTC CA 3') using StuI B plasmid as template to generate megaprimers.
These deletion constructs define two regions as important for CD5 expression: a minimal/core promoter region (HincII to HinfI site) that is required for basal activity and a regulatory region necessary for the full CD5 expression (StuI to HincII) site in resting T cells.