Words similar to stui

Example sentences for: stui

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Each of the megaprimers was purified and subsequently amplified with GL1 primer (5' TGT ATC TTA TGG TAC TGT AAC TG 3'), using StuI B or HincII B plasmid as a template.
To determine which of these potential transcription factor binding sites is responsible for regulating normal CD5 expression, we performed site-directed mutagenesis on the CCAAT (mutC), κE2 (mutK) and Ets (mutE) sites from the StuI B reporter construct.
These deletion constructs define two regions as important for CD5 expression: a minimal/core promoter region (HincII to HinfI site) that is required for basal activity and a regulatory region necessary for the full CD5 expression (StuI to HincII) site in resting T cells.
To determine whether either the CD5X or the CD5Y region can affect the full level of transcription controlled by the 43 bp CD5 regulatory promoter, we generated the mutations described above in the CD5X and CD5Y regions in the StuI B reporter construct.
By constructing a deletion series of CD5 promoter constructs using luciferase as the reporter gene, we demonstrate that a 43 bp region between the StuI and HincII sites at -215 to -172 is necessary for normal, resting CD5 expression level in T cells.