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Example sentences for: stui

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Each of the megaprimers was purified and subsequently amplified with GL1 primer (5' TGT ATC TTA TGG TAC TGT AAC TG 3'), using StuI B or HincII B plasmid as a template.
By constructing a deletion series of CD5 promoter constructs using luciferase as the reporter gene, we demonstrate that a 43 bp region between the StuI and HincII sites at -215 to -172 is necessary for normal, resting CD5 expression level in T cells.
To understand the effect of Ets-1 on CD5 expression, we co-transfected EL4 cells with increasing amounts of Ets-1 expression plasmid (pEVRF-ets-1) along with a StuI B reporter construct containing either the wild type (S) or mutated Ets binding site (E).
To determine whether either the CD5X or the CD5Y region can affect the full level of transcription controlled by the 43 bp CD5 regulatory promoter, we generated the mutations described above in the CD5X and CD5Y regions in the StuI B reporter construct.
However, the deletion of an additional 43 bp (from StuI to HincII site at -172) reduces the reporter activity by more than five-fold to basal level, which is somewhat higher than the background level obtained with a promoterless luciferase construct (GLB = pGLBasic).