Words similar to stss

Example sentences for: stss

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To amplify gene-specific STSs, D. erecta, D. pseudoobscura, D. willistoni and D. littoralis genomic DNA was isolated from adult flies and used as template for PCR with degenerate primers.
After using physical map information, such as sequence-tagged sites (STSs), 20 mouse chromosomes were constructed together with an 'unassigned' chromosome called chromosome UA in Cel2 and chromosome Un in MGSCv3.
The lengths of fosmids sequenced for the eight candidate regions are shown in Table 1. Though we were able to obtain species-specific STSs for the D. willistoni twi gene, we were not able to obtain clones for this region from the D. willistoni fosmid library.
Gaps in the contigs were closed by a combination of DNA sequence alignments of BESs with HTGS data, clone walking using RNA probes from end clones to hybridize to the RPCI-11 gridded filters, and verification of clone positions by PCR for STSs.
We designed degenerate PCR primers for a set of eight well-characterized genes ( apterous (ap), even-skipped (eve), fushi-tarazu (ftz), twist (twi) , and Rhodopsins 1, 2, 3 and 4 ( Rh1, Rh2, Rh3 and Rh4 )) to obtain species-specific sequence-tagged sites (STSs) that were subsequently used for hybridization to gridded fosmid filters (see Materials and methods).