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This construct is expressed at a low enough rate so that the subcellular localization of the Fz protein is still preferentially found along the distal edge of wing cells (Strutt, 2001).
D. Strutt kindly provided flies carrying the arm-fz-GFP transgene [ 10 ] and J. Axelrod kindly provided flies carrying a dsh-GFP transgene (this construct uses the endogenous dsh promoter) [ 12 ] . Temperature shift experiments were done as previously [ 20 ] .