Words similar to strps
Example sentences for: strps
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At 55°C annealing temperature and no PCR multiplexing, few differences were observed between the old and new primer pairs, but under more stringent conditions (60°C annealing temperatures), 79 STRPs were found to amplify better with the new primer pairs (two examples are shown in Figure 2).
Fourteen pentanucleotide STRPs were also included in the combined Sets.
Comparison of the PCR primer sequences for Set 10 STRPs with the new public genomic sequences revealed that a surprisingly high 25% of the STRPs had mismatches in at least one of the primers (an example is shown in Figure 1).
Typing optimal STRPs also leads to lower genotyping costs by providing more information, by reducing the need for duplicate genotyping, by permitting the use of shorter gels (with lower resolving power but shorter run times), and by increasing the efficiency of allele calling.
With one exception on chromosome 6p (see below) genetic map positions for the Screening Set STRPs were taken from the most recent Marshfield map [ 20 ] or by interpolation using the Marshfield map and the genetic and physical map positions described in the previous paragraph.