Example sentences for: streptavidin-coated

How can you use “streptavidin-coated” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Known numbers of biotinylated Ad5.CMV-LacZ particles were incubated in streptavidin-coated wells for immobilization, and the number of viral particles remaining unbound in the solution was estimated from their infectivity on D-17 cells (Fig.

  • This result is in contrast with our previous study, which showed that greater than 95% of biotinylated Ad5.CMV-LacZ, prepared under the same conditions, can be readily captured on streptavidin-coated microbeads [ 10 ] . Thus, the relatively low immobilization efficiency of biotinylated Ad5.CMV-LacZ on the surface of streptavidin-coated wells is not attributable to the absence of accessible biotin moieties on the surfaces of a fraction of viral particles or the presence of unreacted biotinylation reagent in the biotinylated Ad5.CMV-LacZ preparations.

  • These biotinylated adenoviral vectors were analyzed for their ability to infect cells when immobilized on streptavidin-coated solid surfaces.

  • 2above, only about 15 - 40% of biotinylated Ad5.CMV-LacZ applied can actually be immobilized on streptavidin-coated wells.

  • We previously showed that biotin moieties can be attached covalently to the outer surface of adenoviral vectors without appreciable effect on their infectivity under carefully controlled biotinylation conditions [ 10 11 ] . Such biotinylated viral particles could be attached, specifically and stably, to a solid surface on which (strept)avidin is covalently immobilized (avidin- or streptavidin-coated solid surface).


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