Words similar to streptavidin-coated

Example sentences for: streptavidin-coated

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Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and added to streptavidin-coated wells (2.
Known numbers of biotinylated Ad5.CMV-LacZ particles were incubated in streptavidin-coated wells for immobilization, and the number of viral particles remaining unbound in the solution was estimated from their infectivity on D-17 cells (Fig.
Thus, if the infectivity is compared using the actual number of viral particles present in each well, Ad5.CMV-LacZ, immobilized on streptavidin-coated wells, may possess an infectivity equivalent to that of free adenoviral vectors.
Despite the extremely tight streptavidin-biotin interaction, only about 15 - 40% of viral particles were bound to the surface of streptavidin-coated wells under the conditions used where the number of viral particles applied was considerably below saturation of the well surface (estimated by both dimensional and biotin-binding capacities of each well).
The mRNA ELISAs are performed in a similar manner except streptavidin-coated microtiter plates are blocked with PBS containing 2% non fat milk for 1 hr at room temperature, treated with RNaseZap, then coated with biotinylated RNA target (100 ng/well) by incubation for 1 hr at room temperature.