Words similar to streptavidin
Example sentences for: streptavidin
How can you use “streptavidin” in a sentence? Here are some example sentences to help you improve your vocabulary:
Biotinylated mouse Ig, streptavidin/horseradish peroxidase conjugate, diamino benzidine, and Vectastain ABC were from Vector (Burlingame, CA, USA).
Detection antibodies (all from Dako; 1:5,000 for 1 h at room temperature) were mouse-anti-goat Ig, mouse-anti-rat Ig, rabbit-anti-mouse Ig, and porcine-anti-rabbit Ig-HRP [ 66 ] . To visualize all transferred proteins, we used the ECL protein biotinylation labeling modules (RPN 2202, Amersham) and streptavidin alkaline phosphatase (V020402, Amersham) in accordance with the manufacturer's instructions.
Surface-labeled egg proteins were identified on 2D streptavidin immunoblots, and corresponding silver-stained protein spots were cored from a 1.5 mm-thick 2D SDS-PAGE gel that had been run in tandem.
The probe arrays were then stained by adding 80 μl staining solution (100 mM MES, 1 M NaCl, 0.05% Tween-20 with 2 mg/ml acetylated BSA (Sigma, St Louis, MO) and 10 μg/ml streptavidin R-phycoerythrin (Molecular Probes, Portland, OR) for 30 min at 25°C.
Hybridized arrays were washed and stained with phycoerythrin-conjugated streptavidin detection chemistry in an Affymetrix Fluidics station.