Words similar to streptavidin
Example sentences for: streptavidin
How can you use “streptavidin” in a sentence? Here are some example sentences to help you improve your vocabulary:
After blocking non-specific binding sites with 3% BSA, 0.1% tween-20 in PBS, the coverslips were incubated with anti-HA (40 μg/ml) or anti-GFP antibody (40 μg/ml), rabbit anti-mouse IgG (Pierce, 1:200), biotin-conjugated anti-rabbit IgG (Vector Laboratories, 1:200), and streptavidin conjugated with either Alexa 488 or Alexa 594 (Molecular Probes, 1:200).
72), anti-CD25 (7D4) and anti-CD62L/L-selectin (MEL-14), Cy-Chrome and PE-labeled streptavidin were purchased from PharMingen (San Diego, CA).
Biotinylated Ad5.CMV-LacZ, prepared by treatment with 15 μg/ml sulfo-NHS-LC-biotin as above, was diluted in PBST and incubated for immobilization in streptavidin-coated wells (well diameter, 0.64 cm; Reacti-Bind Streptavidin Coated Polystyrene Wells, Pierce Chemical) (50 μl per well) for 2 h at 25°C with shaking on a rotary shaker at 150 rpm.
After extensive washing, the slides were incubated with biotinated donkey anti-goat polyclonal antibody (DAKO) pre-absorbed with rat kidney homogenate [ 15 ] , followed by peroxidase-coupled streptavidin, and the peroxidase visualized by diaminobenzidine as recommended by the kit (LSAB) vendor (DAKO).
Hybridized arrays were washed and stained with phycoerythrin-conjugated streptavidin detection chemistry in an Affymetrix Fluidics station.