Words similar to ste
Example sentences for: ste
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The pheromone response pathway is commonly depicted as a simple, linear transmission of the mating signal from the membrane receptor, Ste2p (for alpha-factor) or Ste3p (for a-factor), to the nuclear effectors, Ste12p and Mcm1p, via a MAPK cascade.
It includes Far1p, a protein necessary for pheromone-induced cell cycle arrest in G1 [ 11 ] , Mpt5p, a protein necessary for recovery from cell cycle arrest [ 12 ] , and Bem1p and Sph1, both of which are necessary for establishment of cell polarity during shmooing and budding [ 13 14 ] . In our protein-interaction map there is no direct interaction between a pheromone receptor (Ste2p or Ste3p) and any component of the heterotrimeric G protein complex (Ste4p/Ste18p/Gpa1p), so NetSearch drew indirect paths through Akr1p, a known inhibitor of signaling in the pheromone pathway [ 15 ] . The predicted network does not include the GTPase Cdc42p (paths were instead drawn preferentially through its cofactor Cdc24p, which physically interacts with Ste4p) or Ste20p, because of missing interactions in the protein-interaction map.
For example, products of Ste18, Ste4, Cdc42, Cdc24, Far1, Bem1, Ste20, Ste5, and other proteins are thought to constitute a complex that has numerous interactions among components, and that mediates many different cellular processes [ 14 28 ] . The complex may coordinate mating pheromone detection with (1) cell cycle arrest via Far1p, (2) MAPK signal transduction via Ste5p and Ste20p, and (3) cell polarity via Bem1p and Far1p (among others) [ 29 30 ] .
0. STE buffer gave background problems possibly because the pH of this buffer tended to decrease as temperature increased whereas SSC and SSPE better retained pH.
Although the protein-interaction dataset we used contained no direct interaction between Ste2p/Ste3p and Ste4p, Ste2p-Ste4p has been shown to interact in a targeted yeast two-hybrid study [ 33 ] .
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