Example sentences for: spike-in

How can you use “spike-in” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The spiked transcripts and their final concentrations in the hybridization cocktails are listed in Table 1. Final concentrations in pmol and parts per million (ppm) for each spiked transcript were computed from the known length of each spike-in, assuming a total mass of 2 μg worm cRNA in a 200 μl hybridization volume, and an average length of 1,000 bases for in vitro transcribed worm cRNAs.

  • The spike-in pool was added into hybridization cocktails in proportion to the UV-quantitated cRNA mass in the hybridization, so as to achieve the desired final concentration of spike-ins.

  • Experiments using 0.1 to 10 μg cRNA per hybridization with corresponding amounts of spike-in transcripts, as well as high and low gain settings on the scanner, indicated that readout saturation (not hybridization saturation) accounted for most of the observed curvature in the spike-in response.

  • The specific hybridization intensity (AD) value for each of the 11 spike-in controls is plotted as a function of transcript frequency in units of transcripts per million.

  • On arrays that are commercially available from Affymetrix, one probe set is tiled to monitor each of the spike-in transcripts.


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