Words similar to spermidine

Example sentences for: spermidine

How can you use “spermidine” in a sentence? Here are some example sentences to help you improve your vocabulary:

The transcript encoding ornithine decar-boxylase (ODC), a known androgen target that catalyzes the rate-limiting step in polyamine biosynthesis [ 26], was induced coordinately with enzymes that catalyze downstream steps in polyamine synthetic pathway (spermine synthase, spermidine/spermine N 1-acetyltransferase, and S-adenosylmethionine decarboxylase) (Figure 5c).
The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.
The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.
Although metabolic genes such as ketohexokinase, adenosine deaminase, and spermidine synthase detected by SSHDD assay per se do not directly activate of EC, it is likely that they could represent a component of the "angiogenic switch".
were incubated in reaction buffer (50 mM Tris-acetate (pH 7.5), 150 mM potassium acetate, 10 mM magnesium acetate and 4 mM spermidine) at 37°C for 2 hr in a 10 μL reaction.