Words similar to spermidine
Example sentences for: spermidine
How can you use “spermidine” in a sentence? Here are some example sentences to help you improve your vocabulary:
The PCR product (2 μg in 50 μl) was added to an in vitro transcription reaction containing Ampliscribe T7 RNA polymerase (Epicentre), RNase inhibitor (GIBCO BRL, Rockville, MD; 5 units), low Mg 2+buffer (30 mM Tris-HCl, pH 8, 7.5 mM DTT, 4.5 mM MgCl 2 , 1.5 mM spermidine), UTP (1.
2 mM spermidine/ 10 μM ATP.
GSPS is a bifunctional enzyme: its synthase domain carries out the ATP-dependent fusion of two glutathione moieties to the polyamine spermidine to form the antioxidant metabolite GSP, while its amidase domain hydrolyzes the amide linkage to release glutathione and spermidine [ 26, 55, 56].
Although metabolic genes such as ketohexokinase, adenosine deaminase, and spermidine synthase detected by SSHDD assay per se do not directly activate of EC, it is likely that they could represent a component of the "angiogenic switch".
The purified PCR product (5 μl) was transcribed in 96-well format by incubation for 2 h at 37°C after the addition of 5 μl of a polymerase reaction cocktail consisting of 2 U T7 RNA polymerase, 4.6 U RNase inhibitor, 10 mM NTPs, 3.5 mM digoxigenin-11-UTP, 40 mM Tris pH 8.0, 6 mM MgCl 2 , 10 mM DTT and 2 mM spermidine.