Words similar to sirna
Example sentences for: sirna
How can you use “sirna” in a sentence? Here are some example sentences to help you improve your vocabulary:
4, panel A) show that cells were substantially depleted of profilin at 200 nM siRNA.
Transfection of monolayer cells with the siRNA and subsequent infection of the cells with RSV were carried out essentially as described [ 25 ] . Interestingly, after designing the siRNA and successfully using it to knock down profilin in rat cells, we realized that the rat profilin sequence (Accession No.
In our routine experiments, therefore, we have used 400 nM siRNA to achieve an essentially profilin-null cell.
The anti-profilin double-stranded RNA (siRNA) of the following sequence were made by Dharmacon http://www.dharmacon.com:
Over the last decade, RNA interference (RNAi), mediated by short interfering double-stranded RNA molecules (siRNA or dsRNA), has been gradually recognized as a major mechanism of post-transcriptional gene silencing (PTGS) in species as diverse as plants, Drosophila, and C. elegans [ 1 ] . Recently, 21-nucleotide long dsRNA molecules corresponding to specific mRNA sequences, when introduced into mammalian cells in culture, have been shown to be highly effective in degrading the cognate mRNAs and thus abrogating the expression of the corresponding proteins [ 2 ] . Although the exact mechanism of PTGS is currently unknown and is an area of intense research, the successful use of this phenomenon in cultured mammalian cells has raised the exciting prospect that it can be used as a simple strategy for phenotypic ablation of mammalian gene function ex vivo without the time-consuming and expensive construction of transgenic animals.
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