Words similar to sirna
Example sentences for: sirna
How can you use “sirna” in a sentence? Here are some example sentences to help you improve your vocabulary:
RNA interference using sequence-specific siRNA can be successfully used to knock down profilin, a major actin-modulating protein of the eukaryotic cell.
In our routine experiments, therefore, we have used 400 nM siRNA to achieve an essentially profilin-null cell.
Post-transcriptional gene silencing using synthetic short interfering RNA (siRNA) molecules has been recently used to ablate specific mRNAs and thus, produce phenotypic mutations in specific genes [ 16 17 ] . We have adopted this technology to knockdown specific gene products in RNA viruses that are obligatory intracellular parasites [ 18 ] . In the present study, we have successfully used a similar strategy to generate phenotypic PP1-deficient P. falciparum parasites.
Sirna began as the biotech startup Ribozyme Pharmaceuticals, which tried to develop ribozymes to treat several conditions, including hepatitis C. A ribozyme is an RNA molecule whose sequence and structure allow it to cleave specific target RNA molecules (see Figure 1).
Interestingly, perfect or near-perfect target complementarity is associated with mRNA degradation [ 24 25 26 ] , similar to the effects of siRNA, whereas imperfect base-pairing is associated with regulation by translational inhibition [ 6 27 ] . Recently, siRNAs with imperfect match to target mRNA were observed to function as translational inhibitors [ 28 ] , suggesting that the type of 21-22 nucleotide RNA-mediated regulation may be largely determined by the quality of target complementarity.
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