Example sentences for: single-stranded

How can you use “single-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The three tetracysteine motifs do not form a stably folded structure on its own, but when combined with the 14 kDa C-terminal domain, forms a stably folded 268 amino acid DNA binding domain (ZD domain) that has higher affinity for single-stranded DNA than the 121 amino acid 14 kDa C-terminal region by itself [ 6 ] . Recent sequence and structural analysis suggests that the 14 kDa domain is evolutionarily related to the three tetracysteine motifs and belongs to the zinc ribbon family [ 7 ] . The ZD domain in E. coli topoisomerase I probably evolved from a domain that binds five Zn(II) originally.

  • As shown in Figure 3a, these two domains had similar affinities for binding to the single-stranded substrate.

  • After elution from the gel slice, the labeled single-stranded oligonucleotides were desalted by centrifugation through a Sephadex G10 spin column.

  • The blots were probed with single-stranded internally labeled DNA probes as described previously [ 16 ] . To monitor RNA loading, membranes were re-probed and hybridized with a 32P-labeled probe encoding 18S ribosomal RNA.

  • Top67 and ZD domains have comparable binding affinities to single-stranded DNA but significantly different affinities for double-stranded DNA


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