Words similar to single-stranded
- single-key
- single-nucleotide
- single-parameter
- single-piece
- single-purpose
- single-step
- single-story
- single-strain
- single-strand
- single-strand-binding
- single-stranded
- single-subject
- single-subunit
- single-survey-response
- single-symbiosis
- single-temperature
- single-tube
- single-unit
- singled
- singles
- singleton
Example sentences for: single-stranded
How can you use “single-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:
First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
These proteins additionally contain a carboxy-terminal RING finger domain [ 13], and some of them also contain a single-stranded nucleic-acid-binding CCCH domain between the amino-terminal domain and the RING finger.
A substrate with both single-stranded and double-stranded regions was constructed to mimic such junction in negatively supercoiled DNA.
Total RNA was purified from dissected nephron segments using Tri Reagent (Molecular Research Center) and reverse-transcribed to single-stranded cDNA using Moloney murine leukemia virus reverse transcriptase and 2.5 μM of random hexamers according to the manufacturer's protocol (GeneAmp RNA PCR kit, Perkin Elmer Cetus, Norwalk, CT).
Removal of negative supercoils from DNA by bacterial type IA topoisomerase involves the following steps: (1) binding of the enzyme to the junction of double-stranded and single-stranded DNA [ 8 ] ; (2) cleavage of a single-strand of DNA near the junction with cleavage sequence preference of a cytosine in the -4 position to form the covalent intermediate [ 9 10 ] ; (3) conformational change of the covalent enzyme-DNA complex to result in physical separation of the 5' phosphate covalently linked to the active tyrosine, and the 3' hydroxyl of the cleaved DNA; (4) passage of the complementary single strand through the break; (5) enzyme conformational change to bring the 5' phosphoryl end back into the proximity of the 3' hydroxyl group of the cleaved DNA; (6) religation of the phosphodiester bond.
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