Words similar to single-stranded
- single-key
- single-nucleotide
- single-parameter
- single-piece
- single-purpose
- single-step
- single-story
- single-strain
- single-strand
- single-strand-binding
- single-stranded
- single-subject
- single-subunit
- single-survey-response
- single-symbiosis
- single-temperature
- single-tube
- single-unit
- singled
- singles
- singleton
Example sentences for: single-stranded
How can you use “single-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:
Two complementary molecules of single-stranded DNA have paired bases that bond with each other and form the well-known double helix structure.
There are two homologous type IA topoisomerases present in E. coli . Topoisomerase III has potent DNA decatenating activity for resolution of plasmid DNA replication intermediates, but much weaker relaxation activity than topoisomerase I [ 17 ] . To exhibit maximal relaxation activity, topoisomerase III requires high temperature (52°C) along with low magnesium and monovalent ion [ 17 18 ] . In contrast, E. coli topoisomerase I was not active in the in vitro assay for resolution of plasmid DNA replication intermediates [ 19 ] . Removal of the C-terminal 49 amino acids from the 653 amino acid topoisomerase III protein resulted in drastic reduction of catalytic activity [ 20 ] . Fusion of the carboxyl-terminal 312 amino acid residues of E. coli topoisomerase I, which includes the entire ZD domain, onto the 605 N-terminal amino acids of topoisomerase III generated a hybrid topoisomerase that has relaxation activity resembling topoisomerase III along with weak decatenating activity [ 21 ] . Although preferring single-stranded DNA as binding substrate, topoisomerase I had been shown to also bind double-stranded DNA [ 22 ] , but there is no data available to indicate which domain in the enzyme is responsible for this interaction.
First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.
ZD domain was found to bind to single-stranded DNA, but not double-stranded DNA, with high affinity.
Previous studies have shown that E. coli DNA topoisomerase I cleavage of single-stranded DNA occurs with selectivity for sites with the C nucleotide base at the - 4 position [ 9 10 ] and that the enzyme preferentially cleaves at junctions of double-stranded and single-stranded DNA [ 8 ] . Several different 5'-end labeled substrates were prepared and used in cleavage assays to compare the cleavage sites selected by Top67 versus topoisomerase I. The results showed that with single-stranded substrates, Top67 also preferred cleavage sites with a C nucleotide base at the -4 position as reported for most of the type IA topoisomerases [ 12 ] . There were some differences from topoisomerase I in the relative distribution of cleavage products among the potential cleavage sites (Figure 4a,4b).
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