Example sentences for: single-stranded

How can you use “single-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The blots were probed with single-stranded internally labeled DNA probes as described previously [ 16 ] . To monitor RNA loading, membranes were re-probed and hybridized with a 32P-labeled probe encoding 18S ribosomal RNA.

  • These proteins are known to cooperate with SbcD, nuclease of the calcineurin-like phosphoesterase superfamily and to degrade dsDNA in the 3' → 5' direction generating ssDNA [ 35 36 ] . It seems likely that RecT/Redβ proteins, at least in certain cases, function in conjunction with the SbcCD-pathway, by utilizing the single-stranded regions generated by the SbcCD nuclease.

  • After elution from the gel slice, the labeled single-stranded oligonucleotides were desalted by centrifugation through a Sephadex G10 spin column.

  • These proteins additionally contain a carboxy-terminal RING finger domain [ 13], and some of them also contain a single-stranded nucleic-acid-binding CCCH domain between the amino-terminal domain and the RING finger.

  • The structure of this 67 kDa domain has been determined by X-ray crystallography to be torus-like, indicating the need for protein conformational change for strand passage to take place after DNA cleavage [ 4 ] . Relaxation activity requires the presence of the Zn(II) binding tetracysteine motifs [ 5 ] found between the 67 kDa N-terminal domain (Top67) and the 14 kDa C-terminal single-stranded DNA binding domain (Figure 1).


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