Example sentences for: single-stranded

How can you use “single-stranded” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • First, pBS-NFATp(391-583) was created using single-stranded pBS-KS +-NFATp(NdeI) for site-directed mutagenesis with two oligonucleotides: a unique in-frame ATG codon contained in an Nde I site was created in place of the codon for amino acid 390 using an oligonucleotide of sequence 5'-CATCTGCAGCATCCATATGACTGCATCCCTC-3' and a stop codon was created in the NFATp cDNA after amino acid 583 using an oligonucleotide of sequence 5'-GTTGAAAGACAATAAACAGAATTCTGCCTGGTCTATG-3'.

  • Transcribed sequences that do not encode proteins, but rather catalytic single-stranded RNAs such as transfer and ribosomal RNAs [ 32], should be treated independently because they are more highly conserved across taxa than messenger RNAs.

  • Two molecules of double-stranded DNA (duplexes) can further associate together if they have complementary single-stranded overhangs (sticky ends).

  • Single-stranded conformational polymorphism (SSCP) analysis

  • The three tetracysteine motifs do not form a stably folded structure on its own, but when combined with the 14 kDa C-terminal domain, forms a stably folded 268 amino acid DNA binding domain (ZD domain) that has higher affinity for single-stranded DNA than the 121 amino acid 14 kDa C-terminal region by itself [ 6 ] . Recent sequence and structural analysis suggests that the 14 kDa domain is evolutionarily related to the three tetracysteine motifs and belongs to the zinc ribbon family [ 7 ] . The ZD domain in E. coli topoisomerase I probably evolved from a domain that binds five Zn(II) originally.


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