Words similar to single-strand
Example sentences for: single-strand
How can you use “single-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:
In any case, telomerase in vitro requires that the 3' primer end protrudes on a single-strand tail (review: [ 9 ] ). Thus another activity is required to resect the 5' underhanging strand (see [ 67 ] ), and this enzyme too would need access to the break.
In the third reaction, only the primer complementary to the sense-orientation of the PCR product was present during RT single-strand synthesis (to assay for sense transcription).
The single-strand DNA substrate designated for the ZD domain in the catalytic mechanism of the enzyme may be the strand of DNA complementary to the strand first cleaved by the enzyme to form the covalent complex.
This method can produce artifacts, however, because it requires the fixation and permeabilization of cells and denaturation of chromatin before hybridization with labeled single-strand probes [ 12].
Studies in Escherichia coli have shown that, although RecA is the principle protein involved in pairing and strand exchange, unrelated proteins, that have a much more restrictive phyletic distribution, can also promote similar reactions in a RecA dependent or RecA-independent manner [ 3 ] . These alternative or additional mediators of homologous recombination include the well-characterized prophage RecT, phage λ Redβ and phage P22 ERF proteins [ 4 5 ] . Similarly, in yeast and vertebrates, the RAD52 protein is involved in the pairing and strand exchange reaction and can promote recombination in a RAD51 (the eukaryotic RecA homolog)-dependent or independent manner [ 6 ] . The RecT protein works in conjunction with the RecE-nuclease [ 7 ] and was initially described in genetic studies on the complemention of mutations in the RecBCD pathway of DNA repair [ 8 9 10 ] . Biochemically, RecT has been shown to bind single-stranded (ss) DNA 3' overhang regions generated by the RecE nuclease, and promote strand exchange between homologous DNA partners by assisting the pairing of complementary single-stranded regions [ 4 10 ] . The reaction catalyzed by the RecT/RecE system is similar to that described for the phage λ exonuclease (exo/Redβ) and the single-strand annealing protein Redβ.
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