Example sentences for: single-strand

How can you use “single-strand” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • The ZD domain interacts with the passing single-strand DNA to be transported (T-strand).

  • Removal of negative supercoils from DNA by bacterial type IA topoisomerase involves the following steps: (1) binding of the enzyme to the junction of double-stranded and single-stranded DNA [ 8 ] ; (2) cleavage of a single-strand of DNA near the junction with cleavage sequence preference of a cytosine in the -4 position to form the covalent intermediate [ 9 10 ] ; (3) conformational change of the covalent enzyme-DNA complex to result in physical separation of the 5' phosphate covalently linked to the active tyrosine, and the 3' hydroxyl of the cleaved DNA; (4) passage of the complementary single strand through the break; (5) enzyme conformational change to bring the 5' phosphoryl end back into the proximity of the 3' hydroxyl group of the cleaved DNA; (6) religation of the phosphodiester bond.

  • Although chromatid breakage requires an endonuclease activity, the cutter, directed by a Cbs-binding activity, no Cbs-dependent "binder/cutter" activity has been detected in vitro . The cutter could be an activity of telomerase itself [ 39 43 ] . Telomerase of both Tetrahymena and Euplotes has a single-strand endonuclease activity [ 45 43 ] . It can trim potential primers back, so that the 3' terminus pairs with template RNA [ 46 ] . Thus, while it may serve a 3' editing function, the existence of a nuclease activity in potential proximity to the site of chromatid breakage is provocative, suggesting that telomerase provides the

  • Single strand PCR probes for Northern hybridization representing 553 bp of 3' UTR or Exon II of mNoc were generated using a modification of the single-strand DNA protocol of Bednarczuk, et al.

  • In the third reaction, only the primer complementary to the sense-orientation of the PCR product was present during RT single-strand synthesis (to assay for sense transcription).


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