Words similar to simplex
Example sentences for: simplex
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Cortical astrocytes were harvested from neonatal C57BL/6 mice and infected with a replication-defective retrovirus encoding Tag and neomycin resistance as previously described [ 13 ] . The transferred viruses were SV40-6 [ 5 ] , in which wild type Tag is expressed from the M-MuLV LTR promoter; SV(X)T K1, which is constructed the same as SV40-6 except that the Tag is mutant [ 38 ] and defective for binding the retinoblastoma protein family [ 39 ] ; Linker-tkT, in which the cDNA for Tag is expressed from the herpes simplex 1 tk promoter [ 11 ] . The differentiation and transformation phenotypes of the cell lines utilizing the M-MuLV LTR promoter have been described [ 6 13 ] . The Linker-tkT lines (tkT) were either isolated as clones after G418 selection (as previously described) [ 6 ] or frozen immediately after selection (pooled clones) and clonally selected at a later time.
Plasmid pVP16-mRXR encodes a fusion protein between the transcriptional transactivator VP16 from Herpes simplex virus (HSV) and the mouse RXR protein.
For instance, viruses including Herpes simplex virus type 1 (HSV-1) have been known to induce severe alterations of the host translational apparatus, including the up-regulation of ribosomal proteins and the progressive association of several nonribosomal proteins, such as VP19C, VP26, and the poly(A)-binding protein 1 (PAB1P) to ribosomes [ 39 ] . In the case of HIV-1, approximately one infectious HIV-1 genome in an infected cell could be transcribed and translated into 50,000 to 100,000 physical particles [ 40 ] . This poses an immense challenge for the virus to be able to transcribe, splice, transport, and translate its RNA into fully packaged virions in a timely fashion.
Barleria lupulina is well known in Thai folk medicine as an antiinflammatory, and is used against snakebites and varicella zoster virus lesions and showed activity against five clinical isolates of herpes simplex virus type 2 [ 57 ] . Compounds found in the leaves of Barleria lupulina are barlerin, acetylbarlerin, shanzhiside methyl ester, acetylshanzhiside methyl ester, ipolamiidoside and iridoid glucosides [ 58 ] . An antibiotic and immunostimulant protein was reported from the plant and other species and patented [ 57 ] .
Stable transfected cell lines containing these constructions were selected by two methods: co-transfection of these plasmids with a second plasmid containing a hygromycin-resistance gene driven by a Herpes simplex thymidine kinase promoter or transfection of GFP-BLM allele plasmids that had been modified to contain the hygromycin-resistance gene expression unit.