Words similar to sigma-
Example sentences for: sigma-
How can you use “sigma-” in a sentence? Here are some example sentences to help you improve your vocabulary:
Wild-type mice expressed cftr protein by immunoblotting, while cftr (-/-) did not [ 14 ] . All chemicals were from Sigma unless otherwise stated (Sigma-Aldrich, St. Louis, MO).
Lysis Buffer contained 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 μg/ml aprotinin, 10 μg/ml leupeptin, 1 mM EDTA, 50 mM NaF, and 1 mM Na orthovanadate (all from Sigma-Aldrich, Inc.).
For detection of phosphotyrosine-containing proteins, filters were blocked with 1% bovine serum albumin (Sigma-Aldrich).
The transgene product was detected in total protein extracts using a mouse monoclonal anti-FLAG antibody (Sigma-Aldrich, St. Louis, MO) diluted 1:1000 with a mouse monoclonal anti-GAPDH antibody (Research Diagnostics Inc., Flanders, NJ) diluted 1:150,000 as a loading control.
Stimulated cells were then resuspended in Lysis Buffer containing 5% nonidet P-40 (NP-40, Sigma-Aldrich, Inc.).
Loading...