Example sentences for: sigma-

How can you use “sigma-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Stimulated cells were then resuspended in Lysis Buffer containing 5% nonidet P-40 (NP-40, Sigma-Aldrich, Inc.).

  • Primers were designed with the Primer3 software http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgiand were synthesized by Sigma-Genosys.

  • Primer NV-2 was biotinylated to facilitate binding of the product to a streptavidin (Sigma-Aldrich) -coated microtiter plate (Immulon II Thermo Labsystems, Franklin, MA, USA).

  • The transgene product was detected in total protein extracts using a mouse monoclonal anti-FLAG antibody (Sigma-Aldrich, St. Louis, MO) diluted 1:1000 with a mouse monoclonal anti-GAPDH antibody (Research Diagnostics Inc., Flanders, NJ) diluted 1:150,000 as a loading control.

  • Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).


How many words do you know? Try our free vocabulary size test!


Search

Search for example sentences

Loading Loading...
Quantcast