Words similar to sigma-

Example sentences for: sigma-

How can you use “sigma-” in a sentence? Here are some example sentences to help you improve your vocabulary:

MSCV/syk expression vectors were packaged into viral particles by transient transfection of 1 × 10 6BOSC cells with 15 μg of plasmid DNA/CaPO 4 precipitates [ 61 ] . BCL 1 .3B3 cells were infected with 500 μl of a 1:2 dilution of viral supernatant in growth medium containing 4 μg/ml polybrene (Sigma-Aldrich, Inc.).
5, 0.5 % Triton X 100) and resuspended in Kinase Buffer - 20 mM Tris-HCl pH 7.5, 5 mM MgCl 2 and 5 mM MnCl 2 (all from Sigma-Aldrich, Inc.).
For cell count experiments, viable cells were enumerated by Trypan Blue exclusion using a hemocytometer (Sigma-Aldrich Inc, St. Louis, MO).
Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).
Cells were then simultaneously fixed with the addition of 830 μl of 0.5% paraformaldehyde in PBS and stained with the addition of 220 μl of 10 μg/ml Hoechst 33342 (Molecular Probes, Inc.) containing 5% Tween-20 (Sigma-Aldrich, Inc) for at least 16 hours at 4°C in the dark.