Example sentences for: sigma-

How can you use “sigma-” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Total RNA from CHO.asbt and BN cells was prepared and analyzed by RNA blotting as previously described [ 17 ] . Cell lysates were prepared by lysing cells in 50 mM Tris-HCl (pH 7.4), 1% NP-40%, 0.25% sodium deoxycholate; 150 mM NaCl, 1 mM EGTA, 1 mM Na 3 VO 4 , 1 mM sodium fluoride, containing a protease inhibitor cocktail (Sigma-Aldrich Canada Ltd.).

  • FLAG-DEK was immunoprecipitated using anti-FLAG resin (Sigma-Aldrich, St Louis, MO, USA) in accordance with the manufacturer's instructions and was eluted by competition with peptide containing three FLAG recognition epitopes.

  • Lysis Buffer contained 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 μg/ml aprotinin, 10 μg/ml leupeptin, 1 mM EDTA, 50 mM NaF, and 1 mM Na orthovanadate (all from Sigma-Aldrich, Inc.).

  • For cell count experiments, viable cells were enumerated by Trypan Blue exclusion using a hemocytometer (Sigma-Aldrich Inc, St. Louis, MO).

  • Agonist 2 was purchased from Sigma-Aldrich (Sigma Aldrich library of rare chemicals.


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