Words similar to sigma-

Example sentences for: sigma-

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5% Tween 20, 1% milk) and incubated with primary antibody for 45 min at 37°C (1:1500 anti-eNOS mono [BD Transduction Laboratories, San Diego, CA, USA]; 1:1500 anti-nNOS poly [Zymed Laboratories, South San Francisco, CA, USA]; 1:200 anti-iNOS poly [Affinity Bioreagents, Golden, CO, USA]; and 1:1500 anti-β-actin mono [Sigma A5441; Sigma-Aldrich, St Louis, MO, USA]).
Cells were then simultaneously fixed with the addition of 830 μl of 0.5% paraformaldehyde in PBS and stained with the addition of 220 μl of 10 μg/ml Hoechst 33342 (Molecular Probes, Inc.) containing 5% Tween-20 (Sigma-Aldrich, Inc) for at least 16 hours at 4°C in the dark.
The total cDNA was directly used as templates for PCR amplification for ATM gene using the primer pairs in Table 2. The primer sequences were based on the ATM sequence (Genbank accession number U33841), and were described for mantle cell lymphoma with slight modification [ 24 ] . The primers were synthesized by Sigma-Genosys (Woodlands, Texas).
Oligonucleotide primers were purchased from Sigma-Genosys; recombinant human VEGF (VEGF 165), bFGF, anti-VEGF (MAB293), and anti-MMP1 (MAB900) from R&D Systems; adult human serum-AB, from Gemini Bioproducts; and bovine skin-derived type I collagen, from Cohesion Inc.
Forskolin, fentanyl, IBMX, hydromorphone, and naltrexone were obtained from Sigma-Aldrich (St.