Words similar to sigma

Example sentences for: sigma

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THP-1 cells were grown in RPMI 1640 media (GIBCO BRL) containing 10% fetal calf serum (FCS) (GIBCO BRL) at 37°C in 5% CO 2 [ 21 ] . To differentiate the THP-1 cells into macrophage-like cells, the cells were treated with 10 μM phorbol myristate acetate (PMA) (Sigma) as follows [ 21 ] . All PMA manipulations were done under low light conditions.
47 g/l, Sigma, St. Louis, MO).
Immediately prior to casting the polony gel, 1.38 μL of JumpStart Taq Polymerase (Sigma), 0.34 μL of 5% APS, and 0.34 μL of TEMED was added to the master mix/primer/DNA solution.
At stage 2, we thawed and cultured 10 7 cells in medium made from a 1:1 mixture of glucose-free DMEM (Invitrogen) and F-12 medium (Invitrogen) containing 0.2% bovine serum albumin (Sigma, St. Louis, Missouri, United States), N2 supplement, 2 μg/ml heparin, 10 ng/ml leukemia inhibitory factor, 20 ng/ml epidermal growth factor, and 20 ng/ml fibroblast growth factor-2.
Ionomycin (Sigma, St. Louis, MO) was added at a concentration of 10 μM at the end of each experiment to obtain the maximal Ca ++elevation for that coverslip.