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Example sentences for: sh-sy

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SH-SY5Y cells are a subclone of the SK-N-SH human neuroblastoma cell line derived from neoplastic neural crest cells and under certain growth conditions, generate neuritic processes [ 12 13 ] . Sternberger and Sternberger [ 14 ] describe SMI-31 mAB as specific for phosphorylated epitopes on the heavy neurofilaments peptide (NF-H) and to a lesser extent medium neurofilament peptide (NF-M).
F98 glioma cells are not known to express neurofilament protein, and SMI-32, a mAB characterized as specific for non-phosphorylated NFs [ 19 ] , stained the expected cytoplasmic network in SH-SY5Y cells (Figure 1C) but did not label any structures in F98 glioma cells (data not shown).
Furthermore, nuclear labeling and DNA synthesis appeared tightly associated in SH-SY5Y cultures, consistent with the suspected role of lamins in DNA replication [ 8 ] . Others have reported SMI-31 labeling in glial as well as neuronal nuclei.
F98 rat glioma cells (ATCC, http://www.atcc.org/, Manassas, VA) were also used for comparative purposes in this study, and were cultured as described for SH-SY5Y cells.
Western analysis using anti-lamin B revealed a similar 70 kDa band in extracts of SH-SY5Y cells, but no bands were labeled by anti-lamin A/C.