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Example sentences for: sh-sy
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SH-SY5Y cells are a subclone of the SK-N-SH human neuroblastoma cell line derived from neoplastic neural crest cells and under certain growth conditions, generate neuritic processes [ 12 13 ] . Sternberger and Sternberger [ 14 ] describe SMI-31 mAB as specific for phosphorylated epitopes on the heavy neurofilaments peptide (NF-H) and to a lesser extent medium neurofilament peptide (NF-M).
Culture dynamics were determined for SH-SY5Y neuroblastoma cells at an initial density of 4.0 × 10 4cells/cm 2. Cells were introduced into five 25 cm 2flasks, then harvested from one flask each day using 5 ml Puck's saline solution (pH 7.3, 137 mM NaCl, 5.4 mM KCl, 0.7 mM Na 2 HPO 4 , 9.5 mM HEPES, 5.6 mM dextrose, and 0.8 mM EDTA).
Furthermore, nuclear labeling and DNA synthesis appeared tightly associated in SH-SY5Y cultures, consistent with the suspected role of lamins in DNA replication [ 8 ] . Others have reported SMI-31 labeling in glial as well as neuronal nuclei.
The RT-97 mAB [ 9 ] has been characterized as recognizing phosphorylated epitopes on the 210 kDa NF-H peptide [ 15 ] , and used similarly to SMI-31 to identify neurites in vitro and in situ [ 16 17 18 ] . One would predict, therefore, that labeling with RT97 would produce staining patterns, including nuclear, similar to those of SMI-31 in SH-SY5Y cells.
[ 12 ] , using the SMI-31 monoclonal antibody (mAB) to identify phosphorylated neurofilament proteins, reported discrete SMI-31 labeling within nuclei of SH-SY5Y neuroblastoma cells.
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