Words similar to sh-sy
Example sentences for: sh-sy
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The RT-97 mAB [ 9 ] has been characterized as recognizing phosphorylated epitopes on the 210 kDa NF-H peptide [ 15 ] , and used similarly to SMI-31 to identify neurites in vitro and in situ [ 16 17 18 ] . One would predict, therefore, that labeling with RT97 would produce staining patterns, including nuclear, similar to those of SMI-31 in SH-SY5Y cells.
Western analysis using anti-lamin B revealed a similar 70 kDa band in extracts of SH-SY5Y cells, but no bands were labeled by anti-lamin A/C.
[ 12 ] , using the SMI-31 monoclonal antibody (mAB) to identify phosphorylated neurofilament proteins, reported discrete SMI-31 labeling within nuclei of SH-SY5Y neuroblastoma cells.
SH-SY5Y cells immunolabeled with SMI-31 mAB or RT-97 mAB demonstrated discrete nuclear localizations in nuclei with pronounced staining in neuronal processes and diffuse staining within cell bodies.
Comparison of nuclear labeling of SH-SY5Y cells immuno-stained with SMI-31 and RT-97 mABs to the culture proliferation index shows that nuclear labeling reached its greatest extent when DNA synthesis was also maximal.
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