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Example sentences for: sh-sy

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Comparison of nuclear labeling of SH-SY5Y cells immuno-stained with SMI-31 and RT-97 mABs to the culture proliferation index shows that nuclear labeling reached its greatest extent when DNA synthesis was also maximal.
Therefore, within the limits of resolution of confocal microscopy, SMI-31 mAB and RT-97 mAB label epitopes in SH-SY5Y cells that are inside the nucleus.
Culture dynamics were determined for SH-SY5Y neuroblastoma cells at an initial density of 4.0 × 10 4cells/cm 2. Cells were introduced into five 25 cm 2flasks, then harvested from one flask each day using 5 ml Puck's saline solution (pH 7.3, 137 mM NaCl, 5.4 mM KCl, 0.7 mM Na 2 HPO 4 , 9.5 mM HEPES, 5.6 mM dextrose, and 0.8 mM EDTA).
In SH-SY5Y cell extracts, the SMI-31 and SMI-32 lanes show strong labeling of bands corresponding to the MW of NF-H and NF-M.
SH-SY5Y cells are a subclone of the SK-N-SH human neuroblastoma cell line derived from neoplastic neural crest cells and under certain growth conditions, generate neuritic processes [ 12 13 ] . Sternberger and Sternberger [ 14 ] describe SMI-31 mAB as specific for phosphorylated epitopes on the heavy neurofilaments peptide (NF-H) and to a lesser extent medium neurofilament peptide (NF-M).