Words similar to sh-sy
Example sentences for: sh-sy
How can you use “sh-sy” in a sentence? Here are some example sentences to help you improve your vocabulary:
F98 glioma cells are not known to express neurofilament protein, and SMI-32, a mAB characterized as specific for non-phosphorylated NFs [ 19 ] , stained the expected cytoplasmic network in SH-SY5Y cells (Figure 1C) but did not label any structures in F98 glioma cells (data not shown).
SH-SY5Y cells were maintained in culture as previously described [ 12 ] . Initial cell density was determined using a hemacytometer (Fisher Scientific, Pittsburgh, PA) and diluted appropriately to maintain a seeding density of 4.0 × 10 4cells/cm 2of surface area.
Furthermore, nuclear labeling and DNA synthesis appeared tightly associated in SH-SY5Y cultures, consistent with the suspected role of lamins in DNA replication [ 8 ] . Others have reported SMI-31 labeling in glial as well as neuronal nuclei.
In SH-SY5Y cell extracts, the SMI-31 and SMI-32 lanes show strong labeling of bands corresponding to the MW of NF-H and NF-M.
SH-SY5Y cells immunolabeled with SMI-31 mAB or RT-97 mAB demonstrated discrete nuclear localizations in nuclei with pronounced staining in neuronal processes and diffuse staining within cell bodies.
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