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Example sentences for: sh-sy

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Furthermore, nuclear labeling and DNA synthesis appeared tightly associated in SH-SY5Y cultures, consistent with the suspected role of lamins in DNA replication [ 8 ] . Others have reported SMI-31 labeling in glial as well as neuronal nuclei.
SMI-31 mAB labeled F98 rat glioma nuclei and cytoplasm with equal or greater avidity than in SH-SY5Y cells (Figure 1D).
Therefore, within the limits of resolution of confocal microscopy, SMI-31 mAB and RT-97 mAB label epitopes in SH-SY5Y cells that are inside the nucleus.
SH-SY5Y cells were maintained in culture as previously described [ 12 ] . Initial cell density was determined using a hemacytometer (Fisher Scientific, Pittsburgh, PA) and diluted appropriately to maintain a seeding density of 4.0 × 10 4cells/cm 2of surface area.
Culture dynamics were determined for SH-SY5Y neuroblastoma cells at an initial density of 4.0 × 10 4cells/cm 2. Cells were introduced into five 25 cm 2flasks, then harvested from one flask each day using 5 ml Puck's saline solution (pH 7.3, 137 mM NaCl, 5.4 mM KCl, 0.7 mM Na 2 HPO 4 , 9.5 mM HEPES, 5.6 mM dextrose, and 0.8 mM EDTA).