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Example sentences for: sh-sy

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The RT-97 mAB [ 9 ] has been characterized as recognizing phosphorylated epitopes on the 210 kDa NF-H peptide [ 15 ] , and used similarly to SMI-31 to identify neurites in vitro and in situ [ 16 17 18 ] . One would predict, therefore, that labeling with RT97 would produce staining patterns, including nuclear, similar to those of SMI-31 in SH-SY5Y cells.
[ 12 ] , using the SMI-31 monoclonal antibody (mAB) to identify phosphorylated neurofilament proteins, reported discrete SMI-31 labeling within nuclei of SH-SY5Y neuroblastoma cells.
SH-SY5Y cells immunolabeled with SMI-31 mAB or RT-97 mAB demonstrated discrete nuclear localizations in nuclei with pronounced staining in neuronal processes and diffuse staining within cell bodies.
Furthermore, nuclear labeling and DNA synthesis appeared tightly associated in SH-SY5Y cultures, consistent with the suspected role of lamins in DNA replication [ 8 ] . Others have reported SMI-31 labeling in glial as well as neuronal nuclei.
F98 rat glioma cells (ATCC, http://www.atcc.org/, Manassas, VA) were also used for comparative purposes in this study, and were cultured as described for SH-SY5Y cells.