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Example sentences for: sevac

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This was followed by peroxidaseconjugated and rat kidney preabsorbed swine anti-mouse IgG (SEVAC, Prague) or goat anti-mouse IgG and IgM (Jackson Immunoresearch), respectively.
Briefly, specimens were washed with phosphate buffered saline (PBS) and incubated 20 minutes with mouse-anti human Thy-1, clone F10-44-1 [ 33 34 ] (5 μg/ml in PBS) reacting with placental villous pericytes, extravillous fibroblasts, and decidual cells [ 15 ] . After extensive washing in PBS, specimens were incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC, Prague, Czech Republic) diluted 1:50 and preabsorbed with rat kidney homogenate [ 15 ] . Antigen-antibody complexes were detected by standard diaminobenzidine technique (brown color).
Specimens were then transferred to room temperature, extensively washed in freshly prepared PBS, pH 7.22, and incubated 20 minutes with swine anti-mouse IgG peroxidase conjugate (SwAM; SEVAC Praha, Prague, Czech Republic) diluted 1:20, and preabsorbed with rat kidney homogenate to remove nonspecific background [ 46 ] . Control slides were similarly processed, but primary antibody was replaced with PBS.