Words similar to serines
Example sentences for: serines
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in vivo, a wild type GSK3β expression vector was cotransfected with either wtp53 or p53 with mutations in serines 33 or 37.
As a comparison, a non-palmitoylated mutant of α q , in which cysteines 9 and 10 have been substituted with serines, is found predominantly in the soluble fraction (Figure 2E, lanes 7 and 8), as described previously [ 13 ] . Surprisingly, in comparison to α i2 , sα i2 was always detected in fewer cells, by immunofluorescence analysis, and showed lower levels of protein expression, by western blot analysis of subcellular fractions (Figure 2E) or whole cell lysates (not shown).
The present data indicates that transcriptional activation of p53 by GSK3β requires both serines 33 and 37, but it does not allow us to determine if phosphorylation of serine 33 is dependent on phosphorylation of serine 37 in vivo.
ATM therefore controls the phosphorylation of serines 15 and 20 of p53.
The triple cysteine mutant (pR-TCM) has all three of its native cysteines (Cys-107, Cys-156, and Cys-175) replaced with serines, and was prepared using the same E. coli expression system and purification methods as the wild type.
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