Example sentences for: sequencing

How can you use “sequencing” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • All sequencing reactions were prepared with the Applied Biosystems, Inc. (ABI) PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit (Applied Biosystems) according to the manufacturer.

  • Cycle sequencing extension products were created in a final volume 20 μl reaction using 8 μl H 2 O, 4 ul forward or reverse primer at 20 ng/μl, 4 μl template DNA (amplicon) at 4 ng/μl for fragments up to 200 bp, 5 ng/μl for fragments between 200 and 400 bp and 6 ng/μl for fragments greater than 400 bp, 2 μl Big Dye Terminator Ready Reaction mix (PE-ABI, Foster City, Ca.)

  • DNA cloning and sequencing

  • These have included the sequencing of randomly selected cDNAs from various tissue sources, the development of computer-based prediction programs of ever-increasing accuracy, and the direct comparison between the human genome and the genome sequences of other vertebrates and invertebrates [ 3 4 5 6 7 8 9 ] . Using these approaches, fully annotated genomes of numerous species will be available within a relatively short time.

  • Using serially mixed dilutions of DNA from NSCLC cell lines (H1975, L858R- and T790M-positive; H-2030, EGFR wild-type) for calibration, this assay detects the presence of the T790M mutation when H1975 DNA comprises 3% or more of the total DNA tested, compared to a sensitivity of 6% for direct sequencing (data not shown).


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