Words similar to separating
Example sentences for: separating
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The lysates were centrifuged at 4000 × g for 15 minutes, then aliquoted and stored at -20°C prior to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western analysis as previously described [ 12 ] . Protein concentrations of the cell lysates were determined using the Bio-Rad Protein Assay (Hercules, CA), and sample volumes adjusted so that 5-10 μg of total protein were loaded into wells of a discontinuous (12% separating, 4% stacking) polyacrylamide gel.
The hyperthermophilic topoisomerase I from Thermotoga maritima has been shown to coordinate one Zn(II) with a unique tetracysteine motif Cys-X-Cys-X 16 -Cys-X-Cys but Zn(II) binding is not required for relaxation activity [ 33 ] . The sequence of this unique tetracysteine motifs is somewhat different from those present in other type IA topoisomerases in that the other tetracysteine motifs always had at least two amino acids separating the pairs of cysteines (Cys-X 2-11 -Cys), instead of just one amino acid (Cys-X-Cys) in T. maritima topoisomerase I [ 33 ] . Therefore the structure and function of the single Zn(II) binding motif in T. maritima may differ from the multiple Zn(II) binding motifs in E. coli topoisomerase I. Direct interaction between DNA and the T. maritima Zn(II) binding motif has not been demonstrated.
After Tony Blair in Washington, the German Chancellor Helmut Kohl has now publicly given his support to the American strategy on Iraq, spectacularly separating himself from France and other members of the European Union."
Then there's Ted and Jane separating.
This modification occurs on a cysteine residue, three residues proximal to the C-terminus with two aliphatic amino acids separating the cysteine from the C-terminus (hence the term CAAX box).
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