Example sentences for: self-ligation

How can you use “self-ligation” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Eco NI (position 817 and 838), followed by Klenow treatment and self-ligation.

  • The KIAA1194 cDNA was first 3'-truncated upon restriction with Bsm I (nt 3642) and Sac II (in pBluescript II SK+ vector), Klenow treatment and self-ligation, to remove undesirable Eco NI restriction sites.

  • Each population of cDNA was ligated into a pBluescript vector that had been digested with BamHI and alkaline phosphatased to decrease the rate of self-ligation.


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