Words similar to self-ligated

Example sentences for: self-ligated

How can you use “self-ligated” in a sentence? Here are some example sentences to help you improve your vocabulary:

To make pBigT, plasmid pSAβgeo was digested with ClaI and XbaI to remove the βgeo and bpA sequence but leave behind the SA, the ends were Klenow filled and the plasmid self-ligated.
Next, pSAβgeo was digested with SacI to remove the SA and βgeo sequences, but leave behind the bpA, and self-ligated.