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Example sentences for: segregants

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Markers segregated 2:2 in nearly all cases and possession of a 1976 bp PCR fragment using primers 4726 and 4722 always correlated with geneticin-resistance while possession of a 1200 bp product correlated with geneticin-sensitivity, as expected for segregants containing a nondisrupted HNT2 gene.
F2 segregants containing only the target locus were identified by PCR based genotyping, analogous to the strategy exemplified in Fig.
Moreover, the suppression of silencing at the E82 locus was partially but not fully heritable given that some but not all E82 segregants lacking L91' were wild-type (Table 3).
This conclusion is supported by additional Southern analyses of Sca I digests and Eco RI/ Pst I digests of genomic DNA from the parental diploid, the heterozygotes, and the 54 Arg +Ura +segregants (data not shown).
Haploid segregants from three complete tetrads were cultured for 24, 48 and 72 hours in SDC medium, lysed, and levels of ApppN and AppppN were determined.