Words similar to segregants

Example sentences for: segregants

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Recombination rates at the UAU1 gene in the heterozygote were determined as described by Enloe et al [ 16 ] . Ura +segregants arose at a rate of 5 x 10 -5per division and Arg +Ura +segregants arose at rate of 8 x 10 -9per division.
This conclusion is supported by additional Southern analyses of Sca I digests and Eco RI/ Pst I digests of genomic DNA from the parental diploid, the heterozygotes, and the 54 Arg +Ura +segregants (data not shown).
We found that 3/26 Arg +Ura +segregants emanating from a CES1/ces1::UAU1 heterozygote were homozygous for disruption at both loci ( ces1::UAU1/ces1::URA3 ) and had lost the wild-type CES1/ZDS1 allele [B.
Such a reactivation had previously been observed in the dihybrid C73/E82 F1 parent plants [ 27 ] . The reactivated expression was subsequently ascribed to the E82 locus, rather than C73, by testing F2 segregants lacking C73 (see Figure 5below).
Markers segregated 2:2 in nearly all cases and possession of a 1976 bp PCR fragment using primers 4726 and 4722 always correlated with geneticin-resistance while possession of a 1200 bp product correlated with geneticin-sensitivity, as expected for segregants containing a nondisrupted HNT2 gene.