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Example sentences for: segregants

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Markers segregated 2:2 in nearly all cases and possession of a 1976 bp PCR fragment using primers 4726 and 4722 always correlated with geneticin-resistance while possession of a 1200 bp product correlated with geneticin-sensitivity, as expected for segregants containing a nondisrupted HNT2 gene.
Moreover, the suppression of silencing at the E82 locus was partially but not fully heritable given that some but not all E82 segregants lacking L91' were wild-type (Table 3).
F2 segregants containing only the target locus were identified by PCR based genotyping, analogous to the strategy exemplified in Fig.
Such a reactivation had previously been observed in the dihybrid C73/E82 F1 parent plants [ 27 ] . The reactivated expression was subsequently ascribed to the E82 locus, rather than C73, by testing F2 segregants lacking C73 (see Figure 5below).
Haploid segregants from three complete tetrads were cultured for 24, 48 and 72 hours in SDC medium, lysed, and levels of ApppN and AppppN were determined.