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Example sentences for: segregants

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Recombination rates at the UAU1 gene in the heterozygote were determined as described by Enloe et al [ 16 ] . Ura +segregants arose at a rate of 5 x 10 -5per division and Arg +Ura +segregants arose at rate of 8 x 10 -9per division.
Haploid segregants from three complete tetrads were cultured for 24, 48 and 72 hours in SDC medium, lysed, and levels of ApppN and AppppN were determined.
F2 segregants containing only the target locus were identified by PCR based genotyping, analogous to the strategy exemplified in Fig.
In addition, hemizygous segregants were distinguished from homozygotes by segregation of the kanamycin resistance gene in their selfed progeny (compare lanes 1-2 with 3-4).
Such a reactivation had previously been observed in the dihybrid C73/E82 F1 parent plants [ 27 ] . The reactivated expression was subsequently ascribed to the E82 locus, rather than C73, by testing F2 segregants lacking C73 (see Figure 5below).