Example sentences for: sds-polyacrylamide

How can you use “sds-polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • 5% SDS-polyacrylamide gels, and transferred to nitrocellulose membrane (Amersham Piscataway, NJ) via semi-dry transfer.

  • Supernatant (4:1 in denaturing loading buffer, boiled 5 minutes) was loaded, separated on 10% denaturing SDS-polyacrylamide gels, and transferred to Immobilon-P membranes.

  • Proteins were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE), using 12.

  • [ 11 ] . Briefly, hyaluronic acid from bovine vitreous humor was added to 15% SDS-polyacrylamide gel at a final concentration of 0.15 mg/ml.

  • Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).


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