Example sentences for: sds-polyacrylamide

How can you use “sds-polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Fifty μg protein samples were heated in SDS-PAGE sample buffer containing β-mercaptoethanol and then electrophoresed on a 10% SDS-polyacrylamide gel.

  • Supernatant (4:1 in denaturing loading buffer, boiled 5 minutes) was loaded, separated on 10% denaturing SDS-polyacrylamide gels, and transferred to Immobilon-P membranes.

  • Labeled protein was subjected to SDS-polyacrylamide gel electrophoresis and autoradiography.

  • Aliquots of media normalized for cell number were denatured by boiling them in the SDS sample buffer and were loaded on 6% SDS-polyacrylamide gels.

  • Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.


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