Words similar to sds-polyacrylamide
Example sentences for: sds-polyacrylamide
How can you use “sds-polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:
GAGs and proteins resolved by SDS-polyacrylamide gel electrophoresis in Tricine/SDS buffer, as instructed by manufacturer (Novex), were detected with specific stains.
Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).
5% SDS-polyacrylamide gels, and transferred to nitrocellulose membrane (Amersham Piscataway, NJ) via semi-dry transfer.
Fifteen (15) μl of the samples were electrophoresed on a 7.5% SDS-polyacrylamide mini-gel for 1 h at 150 V. The proteins were transferred to a PVDF membrane (Amersham Life Sciences, Arlington Heights, IL) using a Mini Trans-blot Cell (Bio Rad Laboratories, Hercules, CA) at 50 mA.
Aliquots of media normalized for cell number were denatured by boiling them in the SDS sample buffer and were loaded on 6% SDS-polyacrylamide gels.