Example sentences for: sds-polyacrylamide

How can you use “sds-polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • GAGs and proteins resolved by SDS-polyacrylamide gel electrophoresis in Tricine/SDS buffer, as instructed by manufacturer (Novex), were detected with specific stains.

  • Samples representing 2 × 10 5cells were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to Immobilion P membranes.

  • About 50 μg of tissue extracts were separated on 10% SDS-polyacrylamide gels and blotted with either the anti-DAZAP1-C antibody (at a 1/2,000 dilution) or the anti-DAZAP1-P antibody (at a 1/5,000 dilution).

  • Samples containing 60 μg of total protein were mixed with an equal volume of 2x SDS-Laemmli sample buffer (100 mM Tris-HCl, pH 6.8, 20% glycerol, 200 mM dithiothreitol, 4% SDS, and 0.2% bromphenol blue) and boiled for 5 min, then resolved using either 12% SDS-polyacrylamide gel electrophoresis.

  • Supernatant (4:1 in denaturing loading buffer, boiled 5 minutes) was loaded, separated on 10% denaturing SDS-polyacrylamide gels, and transferred to Immobilon-P membranes.


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