Example sentences for: sds-polyacrylamide

How can you use “sds-polyacrylamide” in a sentence? Here are some example sentences to help you improve your vocabulary:

  • Protein amounts in each sample were qualitatively compared in CBB dye staining SDS-polyacrylamide gel and normalized before use for western blot analysis.

  • About 50 μg of tissue extracts were separated on 10% SDS-polyacrylamide gels and blotted with either the anti-DAZAP1-C antibody (at a 1/2,000 dilution) or the anti-DAZAP1-P antibody (at a 1/5,000 dilution).

  • Labeled protein was subjected to SDS-polyacrylamide gel electrophoresis and autoradiography.

  • Forty-eight hours after transfection, cells were lysed and membrane and supernatant fractions harvested as described previously [ 8 ] . 10 μg of membrane proteins and normalized volumes of the supernatants were resolved by SDS-polyacrylamide electrophoresis (10%), transferred to nitrocellulose, and probed with a monoclonal antibody to the EE epitope [ 34 ] . The antigen-antibody complexes were visualized with ECL chemiluminescence (Amersham Biosciences, Piscataway, NJ).

  • Ten microliters of non-heated supernatant was used for western blot analysis and Coomassie Brilliant Blue (CBB) staining after SDS-polyacrylamide gel electrophoresis.


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