Words similar to sds-page
Example sentences for: sds-page
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The precipitated proteins were dissolved in SDS-sample buffer, separated by SDS-PAGE and then p27 Kip1and CDK2 were detected by Western blotting.
SDS-PAGE and Coomassie stained gels showed that full-length Ppt1p and Ppt1p (1-504) each constituted more than 95% of the purified protein (Fig.
4-aminopyridine (4-AP); analysis of variance (ANOVA); 8-(4-chlorophenylthio)adenosine-3',5'-cyclic monophosphorothioate (8-CPT-cAMP); 4',4' diisothicyanato-stilbene-2-2'-disulfonic acid (DIDS); bis-(1,3-dibutylbarbituric acid)trimethine oxonol (DiBAC 4 (3)); 5α-dihydrotestosterone (5α-DHT); Dulbecco's modified eagle's medium (DMEM); enhanced chemiluminescence (ECL); ethylenediaminetetraacetic acid (EDTA); fetal bovine serum (FBS); follicle stimulating hormone (FSH); granulosa cells (GC); human chorionic gonadotropin (hCG); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES); 3β-hydroxysteroid dehydrogenase (3β-HSD); 3-isobutyl-1-methylxanthine (IBMX); least significant difference (LSD); luteinizing hormone (LH); mitogen activated protein kinase (MAPK); 1,3-Benzenedicarboxylic acid, 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetrakis [(acetyloxy)methyl] ester (PBFI-AM); phosphate-buffered saline (PBS); proliferating cell nuclear antigen (PCNA); protein kinase A (PKA); sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE); steroidogenic acute regulatory protein (StAR); tris-buffered saline (TBS).
The nuclei were washed, and protein associated with the nuclei as well as protein in the supernatants was solubilized in SDS sample buffer, resolved by SDS-PAGE, and analyzed by protein immunoblotting with anti-HA antibody.
on SDS-PAGE [ 29 ] . Smith and Alpar also demonstrated the reactivity by immunoblotting against the IROMPs using the sera of convalescent patients recovering from Acinetobacter infection sera by Smith and Alpar [ 18 ] .
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