Words similar to sdc-his

Example sentences for: sdc-his

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Transformants were grown for 24, 48 and 72 hours in SDC-his media and intracellular dinucleoside polyphosphate concentrations were determined as above.
At 24, 48 and 72 hours of growth, 50 ml of cells were harvested, cells counted microscopically, lysed, and levels of AppppN and ApppN were determined as described [ 13 ] . Intracellular concentrations of ApppN and AppppN were calculated using 7 × 10 -14l as the volume of a haploid cell [ 50 ] . Cultures of BY71-6c were transformed with plasmids pRS423, pB05, pB32 and pB86 and transformants were selected on SDC-his media.
The SDC-his sample was incubated for 2 hours at 46°C while other samples were incubated for 2 hours at room temperature prior to extraction for determination of dinucleoside polyphosphate concentrations.
After 48 hours of culture, cells were pelleted and resuspended in either YPD media, water, SDC-his media, or the same media supplemented with 2 mM CdCl 2 , 10 mM caffeine or 1 M sorbitol.
Intracellular concentrations of ApppN and AppppN were determined for transformants from cultures in SDC-his media as above.