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Example sentences for: scaled
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Hierarchical clustering of the non-averaged replicate datasets was carried out using the Cluster program, with average linkage using Pearson correlation as the similarity metric [ 20 ] . Clusters were visualized using TreeView [ 20 ] . To assess the degree of overlap among the different methods, the number of features among the top 500 log 2 Cy5/Cy3 ratios was determined for each pair-wise comparison and plotted in Venn Diagrams, with the overlap region scaled to the number of features.
The dye bias can clearly be seen in the unnormalized Cy3 and Cy5 signal distributions shown in green and red respectively in Figure 2. Both global and scaled print-tip lowess and qspline normalizations showed similar signal-distribution comparisons.
In this approach, the mean hybridization intensities (the 'average differences' (AD)) of all probe sets on each array are scaled to an arbitrary, fixed level [ 17].
Constructs for generating the control reagents are available through the American Type Culture Collection (ATCC); accession numbers are given in Table 1. We describe how scaled frequency normalization can be used to estimate message abundance in cRNA, compute a chip sensitivity metric and provide a natural scale for damping spurious signals from below-sensitivity mRNAs.
On Sunday, for example, the liberal Observer , which previously supported the bombing as a prelude to a successful ground campaign, called Sunday for it to be scaled down, deciding that a land war is no longer a practical possibility.
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