Words similar to sca
Example sentences for: sca
How can you use “sca” in a sentence? Here are some example sentences to help you improve your vocabulary:
SCA8 , an untranslated transcript implicated in spinocerebellar ataxia type 8, overlaps the 5' translation and transcription sites of KLHL1 , a gene primarily expressed in the cerebellum [ 12].
We selected 25 Arg +transformants and analyzed them by Southern blotting for integration of the UAU1 cassette into one of the two CaCET1 chromosomal loci to yield the heterozygote CaCET1/cacet1::UAU1 configuration depicted in Figure 1. Briefly, genomic DNA was isolated from the 25 Arg +strains, then digested with Sca I (which cuts neither CaCET1 nor UAU1 ). The digests were resolved by agarose gel-electrophoresis and transferred to membranes, which were probed with a radiolabeled DNA corresponding to the 5' segment of CaCET1 (probe A in Figure 1).
cDNA - DNA complementary to ribonucleic acid (RNA), DNA - deoxyribonucleic acid, PBS - phosphate buffer saline, DEX - dexamethasone, EST - expressed sequence tag, SAGE - serial analysis of gene expression, SCA - single clone analysis, MA - mixed analysis, bp - base pairs, StDev - Standard Deviation, RT-PCR - real-time polymerase chain reaction.
As an example, for a certain cDNA fingerprint the the peak from SCA for restriction enzyme DdeI was determined at 326,5 bp.
In (i) a single clone analysis (SCA) the procedure was applied to only one clone at a time, and yielded accurate fingerprints for certain genes experimentally.
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